IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科
学科主题临床医学
MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells
Li, Sufang1; Chen, Hong1; Ren, Jingyi1; Geng, Qiang1; Song, Junxian1; Lee, Chongyou1; Cao, Chengfu1; Zhang, Jing1; Xu, Ning2
关键词Tissue factor microRNA-223 TNF-alpha Atherosclerosis Thrombogenesis
刊名ATHEROSCLEROSIS
2014-12-01
DOI10.1016/j.atherosclerosis.2014.09.033
237期:2页:514-520
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]MYOCARDIAL-INFARCTION ; CORONARY SYNDROMES ; MECHANISMS ; ATHEROSCLEROSIS ; DISEASE ; PROTEIN ; PLAQUE ; INFLAMMATION ; BIOLOGY ; BINDING
英文摘要

Objective: Atherosclerosis is a chronic inflammatory process, in which vascular endothelial cells (ECs) become dysfunctional owing to the effects of chemical substances, such as inflammatory factor and growth factors. Tissue factor (TF) expression is induced by the above chemical substances in activated ECs. TF initiates thrombosis on disrupted atherosclerotic plaques which plays an essential role during the onset of acute coronary syndromes (ACS). Increasing evidences suggest the important role of microRNAs as epigenetic regulators of atherosclerotic disease. The aim of our study is to identify if microRNA-223 (miR-223) targets TF in ECs. Methods and results: Bioinformatic analysis showed that TF is a target candidate of miR-223. Western blotting analysis revealed that tumor necrosis factor alpha (TNF-alpha) increased TF expression in aorta of C57BL/6J mice and cultured ECs (EA.hy926 cells and HUVEC) after 4 h treatment. In TNF-alpha treated ECs, TF mRNA was also increased measured by real-time PCR. Real-time PCR results showed that miR-223 levels were downregulated in TNF-alpha-treated aorta of C57BL/6J mice and cultured ECs. Transfection of ECs with miR-223 mimic or miR-223 inhibitor modified TF expression both in mRNA and protein levels. Luciferase assays confirmed that miR-223 suppressed TF expression by binding to the sequence of TF 3′-untranslated regions (3′UTR). TF procoagulant activity was inhibited by overexpressing miR-223 with or without TNF-alpha stimulation. Conclusions: MiR-223-mediated suppression of TF expression provides a novel molecular mechanism for the regulation of coagulation cascade, and suggests a clue against thrombogenesis during the process of atherosclerotic plaque rupture. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000346066600052
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59449
专题北京大学第二临床医学院_心血管内科
作者单位1.Karolinska Inst, Dept Med, Stockholm, Sweden
2.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Li, Sufang,Chen, Hong,Ren, Jingyi,et al. MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells[J]. ATHEROSCLEROSIS,2014,237(2):514-520.
APA Li, Sufang.,Chen, Hong.,Ren, Jingyi.,Geng, Qiang.,Song, Junxian.,...&Xu, Ning.(2014).MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells.ATHEROSCLEROSIS,237(2),514-520.
MLA Li, Sufang,et al."MicroRNA-223 inhibits tissue factor expression in vascular endothelial cells".ATHEROSCLEROSIS 237.2(2014):514-520.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Sufang]的文章
[Chen, Hong]的文章
[Ren, Jingyi]的文章
百度学术
百度学术中相似的文章
[Li, Sufang]的文章
[Chen, Hong]的文章
[Ren, Jingyi]的文章
必应学术
必应学术中相似的文章
[Li, Sufang]的文章
[Chen, Hong]的文章
[Ren, Jingyi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。