|Neuropeptide trefoil factor 3 attenuates naloxone-precipitated withdrawal in morphine-dependent mice|
|Wu, Ping1; Shi, Hai-Shui3,4; Luo, Yi-Xiao1; Zhang, Ruo-Xi1; Li, Jia-1; Shi, Jie1; Lu, Lin1,2; Zhu, Wei-Li1|
|关键词||Addiction Trefoil factor 3 Morphine Withdrawal Corticosterone Fos c-fos|
|WOS标题词||Science & Technology|
|类目[WOS]||Neurosciences ; Pharmacology & Pharmacy ; Psychiatry|
|研究领域[WOS]||Neurosciences & Neurology ; Pharmacology & Pharmacy ; Psychiatry|
|关键词[WOS]||CONDITIONED PLACE PREFERENCE ; CORTICOTROPIN-RELEASING-FACTOR ; CHRONIC UNPREDICTABLE STRESS ; C-FOS ; OPIATE WITHDRAWAL ; PARAVENTRICULAR NUCLEUS ; RECOGNITION MEMORY ; RATS ; EXPRESSION ; BRAIN|
The persistence of physical dependence and craving in addicts is considered to contribute to relapse. Increasing evidence indicates that neuropeptide systems are associated with several phases of drug addiction, but little is known about whether the neuropeptide trefoil factor affects withdrawal symptoms.
This study aims to investigate the potential effects of the neuropeptide trefoil factor 3 (TFF3) on naloxone-precipitated withdrawal symptoms in morphine-dependent mice.
Mice received increasing doses of morphine over 3 days. On day 4, the mice were injected with TFF3 (1.0 mg/kg, i.p.) 30 min after the last dose of morphine. Thirty minutes after TFF3 treatment, naloxone (1 mg/kg, i.p.) was injected, and body weight, jumping behavior, wet-dog shakes, and locomotor activity were assessed 30 min later. Naloxone caused significant weight loss and increased jumping behavior and wet-dog shakes in morphine-dependent mice. TFF3 (1.0 mg/kg) reversed these behavioral symptoms caused by morphine withdrawal, suggesting that TFF3 might ameliorate physical dependence associated with opiate addiction. Furthermore, TFF3 pretreatment significantly reduced morphine withdrawal-induced increases in plasma corticosterone and adrenocorticotropic hormone levels. The glucocorticoid receptor agonist RU486 blocked the behavioral effects of TFF3 on morphine withdrawal symptoms. Finally, Fos expression in the medial prefrontal cortex which was decreased during morphine withdrawal was increased by TFF3 pretreatment.
These findings indicate that TFF3 might be a potential therapeutic candidate for opiate addiction by regulating glucocorticoid secretion and neuronal activation in the prefrontal cortex.
|项目编号||81201038 ; 31371140 ; 81201032 ; 81371489 ; YETP0068 ; H2013206010 ; Q2012078 ; 20121323120002 ; 2012ZX09103-301-049 ; 2013ZX09103-003-012|
|资助机构||National Natural Science Foundation of China ; Beijing Higher Education Young Elite Teacher Project ; Natural Science Foundation of Hebei Province ; Youth Foundation of Science and Technology Studies of Hebei Province ; Special Foundation from Ministry of Education of China Specialized Research Foundation for Doctoral Program of College from Ministry of National Education ; National Science and Technology Major Project|
|作者单位||1.Peking Univ, Natl Inst Drug Dependence, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 6, Inst Mental Hlth, Beijing 100191, Peoples R China
3.Hebei Med Univ, Dept Biochem & Mol Biol, Coll Basic Med, Shijiazhuang 050017, Peoples R China
4.Hebei Med Univ, Hebei Key Lab Med Biotechnol, Shijiazhuang 050017, Peoples R China
|Wu, Ping,Shi, Hai-Shui,Luo, Yi-Xiao,et al. Neuropeptide trefoil factor 3 attenuates naloxone-precipitated withdrawal in morphine-dependent mice[J]. PSYCHOPHARMACOLOGY,2014,231(24):4659-4668.|
|APA||Wu, Ping.,Shi, Hai-Shui.,Luo, Yi-Xiao.,Zhang, Ruo-Xi.,Li, Jia-.,...&Zhu, Wei-Li.(2014).Neuropeptide trefoil factor 3 attenuates naloxone-precipitated withdrawal in morphine-dependent mice.PSYCHOPHARMACOLOGY,231(24),4659-4668.|
|MLA||Wu, Ping,et al."Neuropeptide trefoil factor 3 attenuates naloxone-precipitated withdrawal in morphine-dependent mice".PSYCHOPHARMACOLOGY 231.24(2014):4659-4668.|