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学科主题药学
Study of carbohydrate-protein interactions using glyco-QDs with different fluorescence emission wavelengths
Yang, Yang; Cui, Xi-Kai; Zhong, Ming; Li, Zhong-Jun
关键词NMR Glyco-QDs Multi-valent SPR
刊名CARBOHYDRATE RESEARCH
2012-11-01
DOI10.1016/j.carres.2012.08.018
361页:189-194
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic
资助者Natural Science Foundation of China ; State New Drug Innovation ; National Basic Research Program of China ; State Key Laboratory of Natural and Biomimetic Drugs ; Peking University ; Natural Science Foundation of China ; State New Drug Innovation ; National Basic Research Program of China ; State Key Laboratory of Natural and Biomimetic Drugs ; Peking University
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]QUANTUM DOTS ; NANOCRYSTALS ; BINDING ; NANOPARTICLES ; SPECIFICITY ; LECTINS ; DESIGN ; SYSTEM ; COLI ; SIZE
英文摘要

QDs with different fluorescence emission wavelengths were coated with galactose, glucose, and lactose respectively. The formulas of glyco-QDs were determined by NMR and ICP-OES, and the interactions between glyco-QDs and PNA lectin were investigated by SPR. The results showed that multivalent presentation achieved by using QDs as the scaffold is an effective way to enhance the carbohydrate-protein interactions. The K-D for the interaction of PNA with multivalent glyco-QDs is over 3 x 10(6)-fold lower than those with the same free sugars. The specific recognition for the sugar coated on the QDs by lectin is maintained. These sugar-coated QDs could be used as a fluorescent probe to label and identify glycoproteins. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号90713004 ; 20732001 ; 2009ZX09103-044 ; 2009ZX09501-011 ; 2012CB822100
资助者Natural Science Foundation of China ; State New Drug Innovation ; National Basic Research Program of China ; State Key Laboratory of Natural and Biomimetic Drugs ; Peking University ; Natural Science Foundation of China ; State New Drug Innovation ; National Basic Research Program of China ; State Key Laboratory of Natural and Biomimetic Drugs ; Peking University
WOS记录号WOS:000310843700027
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59462
Collection北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yang,Cui, Xi-Kai,Zhong, Ming,et al. Study of carbohydrate-protein interactions using glyco-QDs with different fluorescence emission wavelengths[J]. CARBOHYDRATE RESEARCH,2012,361:189-194.
APA Yang, Yang,Cui, Xi-Kai,Zhong, Ming,&Li, Zhong-Jun.(2012).Study of carbohydrate-protein interactions using glyco-QDs with different fluorescence emission wavelengths.CARBOHYDRATE RESEARCH,361,189-194.
MLA Yang, Yang,et al."Study of carbohydrate-protein interactions using glyco-QDs with different fluorescence emission wavelengths".CARBOHYDRATE RESEARCH 361(2012):189-194.
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