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学科主题基础医学
Comprehensive characterization of protein 4.1 expression in epithelium of large intestine
Zhang, Jingxin1; Yang, Shaomin2; An, Chao3; Wang, Jie1,3; Yan, Hongxia3; Huang, Yumin4; Song, Jinlei1; Yin, Changcheng1; Baines, Anthony J.5; Mohandas, Narla4; An, Xiuli3,6
关键词4.1R 4.1N 4.1B 4.1G Alternative splicing Large intestine Epithelium
刊名HISTOCHEMISTRY AND CELL BIOLOGY
2014-11-01
DOI10.1007/s00418-014-1224-z
142期:5页:529-539
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Microscopy
研究领域[WOS]Cell Biology ; Microscopy
关键词[WOS]REGULATES CELL-ADHESION ; ACTIN-BINDING DOMAIN ; FUNCTIONAL-CHARACTERIZATION ; ALTERED EXPRESSION ; FERM DOMAIN ; GENE FAMILY ; MEMBRANE ; MOUSE ; COMPLEX ; ORGANIZATION
英文摘要

The protein 4.1 family consists of four members, 4.1R, 4.1N, 4.1B and 4.1G, each encoded by a distinct gene. All 4.1 mRNAs undergo extensive alternative splicing. Functionally, they usually serve as adapters that link actin-based cytoskeleton to plasma membrane proteins. It has been reported that 4.1 proteins are expressed in most animal cell types and tissues including epithelial cells and epithelial tissues. However, the expression of 4.1 proteins in large intestine has not been well characterized. In the present study, we performed RT-PCR, western blot and immunohistochemistry analysis to characterize the transcripts, the protein expression and cellular localization of 4.1 proteins in the epithelia of mouse large intestine. We show that multiple transcripts derive from each gene, including eight 4.1R isoforms, four 4.1N isoforms, four 4.1B isoforms and six 4.1G isoforms. However, at the protein level, only one or two major bands were detected, implying that not all transcripts are translated and/or the proteins do not accumulate at detectable levels. Immunohistochemistry revealed that 4.1R, 4.1N and 4.1B are all expressed at the lateral membrane as well as cytoplasm of epithelial cells, suggesting a potentially redundant role of these proteins. Our findings not only provide new insights into the structure of protein 4.1 genes but also lay the foundation for future functional studies.

语种英语
WOS记录号WOS:000343918700006
项目编号81171905 ; 81272187 ; 7112085
资助机构Natural Science Foundation of China ; Beijing National Science Foundation
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59476
专题北京大学基础医学院_生物物理学系
作者单位1.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
2.Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England
3.New York Blood Ctr, Membrane Biol Lab, New York, NY 10065 USA
4.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
6.Zhengzhou Univ, Coll Life Sci, Zhengzhou 450051, Peoples R China
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GB/T 7714
Zhang, Jingxin,Yang, Shaomin,An, Chao,et al. Comprehensive characterization of protein 4.1 expression in epithelium of large intestine[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2014,142(5):529-539.
APA Zhang, Jingxin.,Yang, Shaomin.,An, Chao.,Wang, Jie.,Yan, Hongxia.,...&An, Xiuli.(2014).Comprehensive characterization of protein 4.1 expression in epithelium of large intestine.HISTOCHEMISTRY AND CELL BIOLOGY,142(5),529-539.
MLA Zhang, Jingxin,et al."Comprehensive characterization of protein 4.1 expression in epithelium of large intestine".HISTOCHEMISTRY AND CELL BIOLOGY 142.5(2014):529-539.
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