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学科主题: 公共卫生
题名:
MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion
作者: Xu, Mai1; Wang, Fan3; Gildersleeve, Jeffrey C.4; Achilefu, Samuel1,2
刊名: HYBRIDOMA
发表日期: 2010-08-01
DOI: 10.1089/hyb.2010.0020
卷: 29, 期:4, 页:355-359
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: MONOCLONAL-ANTIBODIES ; HELICOBACTER-PYLORI ; EXPRESSION ; H-TYPE-2 ; TYPE-2 ; CARCINOMAS ; TISSUES
英文摘要:

The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal antibody designated as MAb L9E10. The binding antigen of MAb L9E10 was identified as blood group (BG) H2 antigen using carbohydrate array and erythrocyte agglutination assays. In immunofluorescence study, we found that BG-H2 was expressed on the surfaces of both colon cancer stem cells and their differentiated progeny. In a functional study, we observed that MAb L9E10 inhibited tumor cell migration and invasion at a concentration of 10 mg/mL in vitro. This result suggests that MAb L9E10 could be used to study cancer biology, particularly cancer stem cell biology. In addition, it is potentially useful for studying gastric diseases caused by Helicobacter pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. Finally, MAb L9E10 is an ideal biological reagent for identifying Bombay blood type in which erythrocytes have no BG-H2 antigen expression.

语种: 英语
项目资助者: NIH ; NCI ; Elsa U. Pardee Foundation
WOS记录号: WOS:000280990500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59487
Appears in Collections:北京大学医药卫生分析中心_期刊论文

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作者单位: 1.Washington Univ, Sch Med, Dept Radiol, St Louis, MO 63110 USA
2.Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA
3.Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China
4.NCI, Biol Chem Lab, Ctr Canc Res, Frederick, MD 21701 USA

Recommended Citation:
Xu, Mai,Wang, Fan,Gildersleeve, Jeffrey C.,et al. MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion[J]. HYBRIDOMA,2010,29(4):355-359.
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