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MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion
Xu, Mai1; Wang, Fan3; Gildersleeve, Jeffrey C.4; Achilefu, Samuel1,2
刊名HYBRIDOMA
2010-08-01
DOI10.1089/hyb.2010.0020
29期:4页:355-359
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]MONOCLONAL-ANTIBODIES ; HELICOBACTER-PYLORI ; EXPRESSION ; H-TYPE-2 ; TYPE-2 ; CARCINOMAS ; TISSUES
英文摘要

The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal antibody designated as MAb L9E10. The binding antigen of MAb L9E10 was identified as blood group (BG) H2 antigen using carbohydrate array and erythrocyte agglutination assays. In immunofluorescence study, we found that BG-H2 was expressed on the surfaces of both colon cancer stem cells and their differentiated progeny. In a functional study, we observed that MAb L9E10 inhibited tumor cell migration and invasion at a concentration of 10 mg/mL in vitro. This result suggests that MAb L9E10 could be used to study cancer biology, particularly cancer stem cell biology. In addition, it is potentially useful for studying gastric diseases caused by Helicobacter pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. Finally, MAb L9E10 is an ideal biological reagent for identifying Bombay blood type in which erythrocytes have no BG-H2 antigen expression.

语种英语
WOS记录号WOS:000280990500013
资助机构NIH ; NCI ; Elsa U. Pardee Foundation
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59487
专题北京大学医药卫生分析中心
作者单位1.Washington Univ, Sch Med, Dept Radiol, St Louis, MO 63110 USA
2.Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA
3.Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China
4.NCI, Biol Chem Lab, Ctr Canc Res, Frederick, MD 21701 USA
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Xu, Mai,Wang, Fan,Gildersleeve, Jeffrey C.,et al. MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion[J]. HYBRIDOMA,2010,29(4):355-359.
APA Xu, Mai,Wang, Fan,Gildersleeve, Jeffrey C.,&Achilefu, Samuel.(2010).MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion.HYBRIDOMA,29(4),355-359.
MLA Xu, Mai,et al."MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion".HYBRIDOMA 29.4(2010):355-359.
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