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Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor
Wang, Yue; Xue, Wei Cheng; Liu, Vincent W. S.; Ngan, Hextan Y. S.
关键词mitochondrial DNA alterations endometrial cancer laser-captured micro-dissection
刊名MITOCHONDRION
2007-02-01
DOI10.1016/j.mito.2006.11.014
7期:1-2页:171-175
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Genetics & Heredity
研究领域[WOS]Cell Biology ; Genetics & Heredity
关键词[WOS]SOMATIC MUTATIONS ; OXIDATIVE STRESS ; HIGH-FREQUENCY ; CARCINOMAS ; SELECTION ; CANCER ; MUTANT ; GENOME ; MTDNA
英文摘要

Somatic mitochondrial DNA (mtDNA) alterations including point mutations and microsatellite instability (MSI) have been frequently detected in human cancers. To further explore the extensiveness of mtDNA alterations, we have analyzed the occurrence of somatic mtDNA mutations in different populations of endometrial cancer cells from the same tumor tissues as compared with adjacent non-tumor cells. Laser-captured micro-dissection was used to harvest endometrial cancer cells from separated areas of the same tumor and adjacent normal cells. Total DNA isolated from micro-dissected cells was PCR amplified and analyzed for mtDNA alterations by polyacrylamide gel electrophoresis and DNA sequencing. Multiple mtDNA alterations were detected in different portions of the same tumor. Different populations of endometrial cancer cells carried different patterns of mtDNA mutations. Interestingly, unlike previous reports, most mutations were found to be heteroplasmic. We have demonstrated the occurrence of hyper-variability of mtDNA alterations in a single piece of tumor tissue. Our observations support the hypothesis that the accumulation of mtDNA alterations is random and expands independently. The data presented here showed the heterogeneity of cancer cells in terms of mtDNA alterations in endometrial cancer. (c) 2006 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

语种英语
WOS记录号WOS:000245701400026
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59494
专题北京大学第二临床医学院
作者单位1.Univ Hong Kong, Dept Obstet & Gynecol, Fac Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Pathol, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Yue,Xue, Wei Cheng,Liu, Vincent W. S.,et al. Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor[J]. MITOCHONDRION,2007,7(1-2):171-175.
APA Wang, Yue,Xue, Wei Cheng,Liu, Vincent W. S.,&Ngan, Hextan Y. S..(2007).Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor.MITOCHONDRION,7(1-2),171-175.
MLA Wang, Yue,et al."Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor".MITOCHONDRION 7.1-2(2007):171-175.
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