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学科主题: 临床医学
题名:
Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor
作者: Wang, Yue; Xue, Wei Cheng; Liu, Vincent W. S.; Ngan, Hextan Y. S.
关键词: mitochondrial DNA alterations ; endometrial cancer ; laser-captured micro-dissection
刊名: MITOCHONDRION
发表日期: 2007-02-01
DOI: 10.1016/j.mito.2006.11.014
卷: 7, 期:1-2, 页:171-175
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Genetics & Heredity
研究领域[WOS]: Cell Biology ; Genetics & Heredity
关键词[WOS]: SOMATIC MUTATIONS ; OXIDATIVE STRESS ; HIGH-FREQUENCY ; CARCINOMAS ; SELECTION ; CANCER ; MUTANT ; GENOME ; MTDNA
英文摘要:

Somatic mitochondrial DNA (mtDNA) alterations including point mutations and microsatellite instability (MSI) have been frequently detected in human cancers. To further explore the extensiveness of mtDNA alterations, we have analyzed the occurrence of somatic mtDNA mutations in different populations of endometrial cancer cells from the same tumor tissues as compared with adjacent non-tumor cells. Laser-captured micro-dissection was used to harvest endometrial cancer cells from separated areas of the same tumor and adjacent normal cells. Total DNA isolated from micro-dissected cells was PCR amplified and analyzed for mtDNA alterations by polyacrylamide gel electrophoresis and DNA sequencing. Multiple mtDNA alterations were detected in different portions of the same tumor. Different populations of endometrial cancer cells carried different patterns of mtDNA mutations. Interestingly, unlike previous reports, most mutations were found to be heteroplasmic. We have demonstrated the occurrence of hyper-variability of mtDNA alterations in a single piece of tumor tissue. Our observations support the hypothesis that the accumulation of mtDNA alterations is random and expands independently. The data presented here showed the heterogeneity of cancer cells in terms of mtDNA alterations in endometrial cancer. (c) 2006 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

语种: 英语
WOS记录号: WOS:000245701400026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59494
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Univ Hong Kong, Dept Obstet & Gynecol, Fac Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Pathol, Beijing 100871, Peoples R China

Recommended Citation:
Wang, Yue,Xue, Wei Cheng,Liu, Vincent W. S.,et al. Detection of mosaic pattern of mitochondrial DNA alterations in different populations of cells from the same endometrial tumor[J]. MITOCHONDRION,2007,7(1-2):171-175.
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