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学科主题: 药学
题名:
Rapid screening and identification of compounds with DNA-binding activity from Folium Citri Reticulatae using on-line HPLC-DAD-MSn coupled with a post column fluorescence detection system
作者: Fu, Qingrong1; Zhang, Cangman1; Lin, Zongtao2; Sun, Hongyang1; Liang, Yi1; Jiang, Haixiu1; Song, Zhiling1; Wang, Hong1; Chen, Shizhong1
关键词: DNA-binding activity ; Feruloylhexaric acids ; Flavonoids ; Folium Citri Reticulatae ; HPLC-DAD-MSn-EB-DNA-FLD ; p-Coumaroylhexaric acids
刊名: FOOD CHEMISTRY
发表日期: 2016-02-01
DOI: 10.1016/j.foodchem.2015.07.009
卷: 192, 页:250-259
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
研究领域[WOS]: Chemistry ; Food Science & Technology ; Nutrition & Dietetics
关键词[WOS]: TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; ETHIDIUM-BROMIDE ; ARRAY DETECTION ; NUCLEIC-ACIDS ; FLAVONOIDS ; LEAVES ; PROBE ; ANTIOXIDANTS ; COMBINATION
英文摘要:

To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MSn-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MSn fragments and DNA-binding activity profile of various components in Folium Cirri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2014ZX09304307-001-011
项目资助者: National Science and Technology Major Project: Safety Testing Technology of the New Drug of TCM and the Standard Research
WOS记录号: WOS:000362304500032
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59507
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Univ Tennessee, Ctr Hlth Sci, Dept Pharmaceut Sci, Memphis, TN 38163 USA

Recommended Citation:
Fu, Qingrong,Zhang, Cangman,Lin, Zongtao,et al. Rapid screening and identification of compounds with DNA-binding activity from Folium Citri Reticulatae using on-line HPLC-DAD-MSn coupled with a post column fluorescence detection system[J]. FOOD CHEMISTRY,2016,192:250-259.
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