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学科主题: 临床医学
题名:
Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis
作者: Zhang Hong1; Feng Jie1; Chang Xiao-hong1; Li Zhong-xing2; Wu Xiao-yi2; Cui Heng1
关键词: endometriosis ; proteomics ; surface-enhanced laser desorption/ionization ; mass spectrometry
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2009-02-20
DOI: 10.3760/cma.j.issn.0366-6999.2009.04.003
卷: 122, 期:4, 页:373-376
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; NASOPHARYNGEAL CARCINOMA ; PROTEOMIC PATTERNS ; CANCER ; SERUM ; DIAGNOSIS ; PREDICTION ; DISCOVERY
英文摘要:

Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).

Methods Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS.

Results After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P <0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three biomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%.

Conclusions SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.

语种: 英语
WOS记录号: WOS:000263739000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59513
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing DeYi Biol Med Technol Co Ltd, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Gynecol Oncol, Beijing 100044, Peoples R China

Recommended Citation:
Zhang Hong,Feng Jie,Chang Xiao-hong,et al. Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis[J]. CHINESE MEDICAL JOURNAL,2009,122(4):373-376.
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