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学科主题: 公共卫生
题名:
Let-7g is involved in doxorubicin induced myocardial injury
作者: Fu, Jun; Peng, Cike; Wang, Wanyi; Jin, Haifeng; Tang, Qiuqiong; Wei, Xuetao
关键词: Doxorubicin ; Let-7g ; miRNA ; Cardiac injury
刊名: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
发表日期: 2012-03-01
DOI: 10.1016/j.etap.2011.12.023
卷: 33, 期:2, 页:312-317
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: INDUCED CARDIOTOXICITY ; CARDIAC-HYPERTROPHY ; MICRORNAS ; INHIBITION ; HEART ; RATS
英文摘要:

Objectives: To investigate whether let-7g (miRNA) was involved in doxorubicin-induced cardiotoxicity.

Methods: Rats were treated with doxorubicin at increasing doses (0 mg/kg, 6 mg/kg, 12 mg/kg, 18 mg/kg). Heart rate, pulse pressure and plasma cardiac troponin T concentrations were measured. Primary cultured myocardial cells were incubated with DOX at increasing concentrations (0 mu mol/1, 0.004 mu mol/1, 0.02 mu mol/1, 0.1 mu mol/1, 0.5 mu mol/1) for 24h. Cellular viability and the beat frequency were measured. For both rats and cultured cells, miRNA content was measured by real-time reverse-transcription PCR.

Results: All DOX-treated rats had a decrease in heart rate, an increase in pulse pressure compared with control group after injections (p < 0.05). Concentration of cTnT was increased significantly in 18 mg/kg group. Content of let-7g decreased significantly (p < 0.05) in 18 mg/kg group in vivo and all the doxorubicin treated group in vitro.

Conclusions: The down regulation of let-7g in the myocardial-injury model suggests that let-7g may play an important role in the development of cardiac disease. (C) 2012 Published by Elsevier B.V.

语种: 英语
WOS记录号: WOS:000301475000024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59514
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Peking Univ, Dept Toxicol, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Fu, Jun,Peng, Cike,Wang, Wanyi,et al. Let-7g is involved in doxorubicin induced myocardial injury[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2012,33(2):312-317.
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