|Arsenic trioxide downregulates the expression of annexin II in bone marrow cells from patients with acute myelogenous leukemia|
|Zhang Xiao-hui1; Hu Yu1; Bao Li2; Jiang Qian2; Yang Ling-hua3; Lu Xi-jing2; Hong Mei1; Xia Ling-hui1; Guo Tao1; Shen Guan-xin4; Zhu Hong-hu2; Zhao Ting2; Song Shan-jun1|
|关键词||arsenic trioxide annexin II acute myelogenous leukemia|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||ACUTE PROMYELOCYTIC LEUKEMIA ; PLASMINOGEN-ACTIVATOR ; RETINOIC ACID ; SURFACE ; CORECEPTOR ; POLYMERASE ; APOPTOSIS ; PROTEINS ; LINES|
Background Most patients with acute myelogenous leukemia (AML) suffer from disordered hemostasis. We have previously shown that annexin II (Ann II), a high-affinity co-receptor for plasminogen/tissue plasminogen activator, plays a central role in primary hyper fibrinolysis in patients with acute promyelocytic leukemia (APL). The expression of Ann II in cells from patients with major subtypes of AML and the effect of arsenic trioxide (As(2)O(3)) on Ann II expression in AML cells were investigated to determine whether As(2)O(3)-mediated downregulation of Ann II could restore hemostatic stability.
Methods A total of 103 patients (48 females and 55 males; age, 19-58 years) were included. Plasma samples were collected before and after treatment as well as after complete remission. Ann II and plasminogen activation were measured in leukemic cells during treatment with 1 mu mol/L As(2)O(3).
Results Before As(2)O(3) treatment, Ann II mRNA expression (real-time PCR) was the highest in M3 cells (P <0.05), higher in M5 cells than that in M1, M2, M4, and M6 cells (P<0.001), and positively correlated with Ann II protein expression (flow cytometry) (r=0.752, P<0.01). Exposure for up to 120 hours to As(2)O(3) (1 mu mol/L) had no significant effect on Ann II protein in M1 and M2 leukemic cells, but decreased Ann II protein expression twofold within 48 hours of exposure in M3 cells (P <0.05) and twofold within 96 hours in M5 cells (P <0.05). The rate of plasmin generation was higher in APL, M5, and M4 cells than in M1, M2, and M6 cells.
Conclusions As(2)O(3) may reduce hyperfibrinolysis in AML by downregulation of Ann II. Furthermore, As(2)O(3) affects more than one form of AML (APL, M4 and M5), suggesting its potential role in their management. Chin Med J 2009;122(17):1969-1973
|作者单位||1.Huazhong Univ Sci & Technol, Dept Hematol, Union Hosp, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China|
2.Peking Univ, Peoples Hosp, Inst Hematol, Beijing 100044, Peoples R China
3.Shanxi Med Univ, Dept Hematol, Clin Med Coll 2, Taiyuan 030001, Shanxi, Peoples R China
4.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Immunol, Wuhan 430022, Hubei, Peoples R China
|Zhang Xiao-hui,Hu Yu,Bao Li,et al. Arsenic trioxide downregulates the expression of annexin II in bone marrow cells from patients with acute myelogenous leukemia[J]. CHINESE MEDICAL JOURNAL,2009,122(17):1969-1973.|
|APA||Zhang Xiao-hui.,Hu Yu.,Bao Li.,Jiang Qian.,Yang Ling-hua.,...&Song Shan-jun.(2009).Arsenic trioxide downregulates the expression of annexin II in bone marrow cells from patients with acute myelogenous leukemia.CHINESE MEDICAL JOURNAL,122(17),1969-1973.|
|MLA||Zhang Xiao-hui,et al."Arsenic trioxide downregulates the expression of annexin II in bone marrow cells from patients with acute myelogenous leukemia".CHINESE MEDICAL JOURNAL 122.17(2009):1969-1973.|