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学科主题: 基础医学
题名:
Characteristics of TCR alpha beta(+)CD4(-)CD8(-) thymocytes in fetal thymic organ culture
作者: Xiao, SY; Li, Y; Qian, XP; Wang, YD; Chen, WF
关键词: FTOC ; TCR alpha beta(+)CD4(-)8(-) thymocyte
刊名: PROGRESS IN NATURAL SCIENCE
发表日期: 2003-06-01
卷: 13, 期:6, 页:419-423
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]: Materials Science ; Science & Technology - Other Topics
关键词[WOS]: T-CELL RECEPTOR ; MURINE THYMOCYTES ; EXPRESSION
英文摘要:

TCRalphabeta+ CD4(-) CD8(-) (TCR+ DN) thymocytes at different developmental periods, i. e. after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb significantly promoted proliferation of TCRalphabeta(+) DN cells generated after 18 days of culture in FTOC, whereas the cells generated after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRalphabeta(+) DN cells at day 9 of FTOC to differentiate into TCRalphabeta(+) CD4(+) /CD8(+) SP cells without detectable transitional stage of TCRalphabeta(+) CD4(+) CD8(+) (DP) cells. In contrast, fewer TCRalphabeta(+) DN cells generated after 18 days of FTOC were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRalphabeta(+) DN cells. In addition, TCRalphabeta(+) DN cells were shown to be less susceptible to apoptosis compared with the other major thymocyte subsets. Taken together, these data have provided insight into the characteristics of TCRalphabeta(+) DN thymocytes.

语种: 英语
WOS记录号: WOS:000183347000004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59542
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Xiao, SY,Li, Y,Qian, XP,et al. Characteristics of TCR alpha beta(+)CD4(-)CD8(-) thymocytes in fetal thymic organ culture[J]. PROGRESS IN NATURAL SCIENCE,2003,13(6):419-423.
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