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A novel fungus concentration-dependent rat model for acute invasive fungal rhinosinusitis: an experimental study
Yan, Yuyan1; Zhao, Zuotao2; Wan, Hongfei1; Wu, Ruochen1; Fang, Jugao3; Liu, Honggang1
关键词Acute invasive fungal rhinosinusitis Animal model Aspergillus fumigatus Mast cells
刊名BMC INFECTIOUS DISEASES
2014-12-20
DOI10.1186/s12879-014-0713-y
14
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases
研究领域[WOS]Infectious Diseases
关键词[WOS]PULMONARY ASPERGILLOSIS ; MAST-CELLS ; NEUTROPENIC RATS ; MURINE MODEL ; SINUSITIS ; CYCLOPHOSPHAMIDE ; CASPOFUNGIN ; BACTERIAL ; PHARMACOKINETICS ; COMPLICATIONS
英文摘要

Background: Acute invasive fungal rhinosinusitis is a lethal infectious process afflicting immunocompromised individuals. Knowledge about this disease is still limited due to the scarcity of animal models designed to study the pathogenesis of this infection. Mast cells are tissue-resident immune cells that participate in a variety of allergic and inflammatory conditions. Limited attention has been given to the role of mast cells in acute invasive fungal rhinosinusitis. Therefore, the objectives of this study were to create a rat model of acute invasive fungal rhinosinusitis based on analyzing the impact of different fungal concentrations on establishing infection, and to observe the changes of mast cells in rats with this disease.

Methods: Sprague-Dawley rats were divided randomly into four groups, three of which were experimental and received different concentrations of Aspergillus fumigatus inoculations, and one was a control group (D). The inoculated Aspergillus fumigatus concentrations were 5 x 10(7) conidia/ml in group A, 10(7) conidia/ml in group B, and 10(6) conidia/ml in group C. Before fungal inoculation, rats were immunosuppressed using cyclophosphamide and cortisone acetate, and had Merocel sponges inserted into the right nares. Hematology and histopathology investigations were then performed.

Results: An acute invasive fungal rhinosinusitis rat model was established successfully with an incidence rate of 90% in group A, 50% in group B and 10% in group C. Aspergillus fumigatus invasion was observed in 20% of the lungs in group A, but was not seen in the remaining groups. In addition, no fungi invaded the orbital tissue, brains, livers, spleens or kidneys of any rat. Compared with the control set, the total number of mast cells in the experimental groups was not significantly increased, but mast cell degranulation, on the other hand, was only found in infected nasal cavities.

Conclusions: This investigation illustrates that various fungal concentrations have different effects on the incidence of acute invasive fungal rhinosinusitis, and it also demonstrates the feasibility of using this model to study the process of fungal rhinosinusoidal invasion. In addition, the results suggest that mast cells may play a role in the protection of sinuses against acute Aspergillus fumigatus infection and in the clearance of established hyphal masses.

语种英语
WOS记录号WOS:000352128600001
项目编号81070769
资助机构Fungi and Fungal Disease Research Center of Peking University ; Department of Pathology, Beijing Tongren Hospital ; National Natural Science Foundation of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59551
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Capital Med Univ, Affiliated Beijing Tongren Hosp, Dept Pathol, Beijing 100730, Peoples R China
2.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
3.Capital Med Univ, Affiliated Beijing Tongren Hosp, Dept ENT, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Yan, Yuyan,Zhao, Zuotao,Wan, Hongfei,et al. A novel fungus concentration-dependent rat model for acute invasive fungal rhinosinusitis: an experimental study[J]. BMC INFECTIOUS DISEASES,2014,14.
APA Yan, Yuyan,Zhao, Zuotao,Wan, Hongfei,Wu, Ruochen,Fang, Jugao,&Liu, Honggang.(2014).A novel fungus concentration-dependent rat model for acute invasive fungal rhinosinusitis: an experimental study.BMC INFECTIOUS DISEASES,14.
MLA Yan, Yuyan,et al."A novel fungus concentration-dependent rat model for acute invasive fungal rhinosinusitis: an experimental study".BMC INFECTIOUS DISEASES 14(2014).
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