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Doxycycline-regulated co-expression of GDNF and TH in PC12 cells
Wang, JJ; Zhang, T; Niu, DB; Wang, K; Li, KR; Xue, B; Wang, XM
关键词adeno-associated virus tyrosine hydroxylase glial cell line-derived neurotrophic factor doxycycline PC12 cell line
刊名NEUROSCIENCE LETTERS
2006-06-19
DOI10.1016/j.neulet.2006.03.004
401期:1-2页:142-145
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]MEDIATED GENE-TRANSFER ; PARKINSONS-DISEASE ; TYROSINE-HYDROXYLASE ; NIGROSTRIATAL SYSTEM ; NEUROTROPHIC FACTOR ; MPTP TOXICITY ; MODEL ; THERAPY ; BRAIN ; AAV
英文摘要

Current gene therapy models for Parkinson′s disease (PD) have adapted two treatment strategies. One is to restore dopamine (DA) production by delivering the genes of DA-synthesizing enzymes such as tyrosine hydroxylase (TH) to the striatum to relieve motor symptoms of PD. Another is to block or slow down progressive degenerative changes by delivering neurotrophic factors such as glial cell line-derived neurotrophic factor (GDNF) to protect the remained neurons. To test the assumption that the combination of the two strategies may have a compound or synergistic effect, we had constructed tetracycline-inducible (tet-off) AAV vector carrying GDNF and TH. After co-transfection of PC 12 cells with this vector and the inducer plasmid, the expression of GDNF and TH protected these cells from 1-methyl-4-phenyl-pyridinium-induced injury, and significantly increased the content of dopamine in GDNF/TH-expressing cells compared with the control. Furthermore, mRNA expression of GDNF and TH could be effectively and reversibly regulated by doxycycline (Dox) and the function of GDNF and TH could be repressed by Dox. These results suggest that the tet-off AAV vector carrying GDNF and TH may be a useful tool for gene therapy in the treatment of PD. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000238082400028
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59552
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Capital Univ Med Sci, Dept Physiol, Beijing 100054, Peoples R China
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GB/T 7714
Wang, JJ,Zhang, T,Niu, DB,et al. Doxycycline-regulated co-expression of GDNF and TH in PC12 cells[J]. NEUROSCIENCE LETTERS,2006,401(1-2):142-145.
APA Wang, JJ.,Zhang, T.,Niu, DB.,Wang, K.,Li, KR.,...&Wang, XM.(2006).Doxycycline-regulated co-expression of GDNF and TH in PC12 cells.NEUROSCIENCE LETTERS,401(1-2),142-145.
MLA Wang, JJ,et al."Doxycycline-regulated co-expression of GDNF and TH in PC12 cells".NEUROSCIENCE LETTERS 401.1-2(2006):142-145.
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