|In vivo induction of T-cell hyporesponsiveness and alteration of immunological cells of bone marrow grafts using granulocyte colony-stimulating factor|
|Jun, HX; Jun, CY; Yu, ZX|
|关键词||bone marrow grafts granulocyte colony-stimulating factor co-stimulatory molecules dendritic cells T cell|
|WOS标题词||Science & Technology|
|关键词[WOS]||VERSUS-HOST-DISEASE ; BLOOD MONONUCLEAR-CELLS ; G-CSF ; DENDRITIC CELLS ; MEDIATED SUPPRESSION ; CYTOKINE PRODUCTION ; MOBILIZED BLOOD ; STEM-CELLS ; TRANSPLANTATION ; PROLIFERATION|
Background and Objectives. Granulocyte colony-stimulating factor (6-CSF)-primed bone marrow (G-BM) transplantation is associated with a low incidence of graft-versus-host disease (GVHD) and has been used successfully in patients with human leukocyte antigen (HLA) matched/mismatched donors. This study evaluated the function of T cells and the quantities of immunological cells of G-BM.
Design and Methods. Bone marrow was obtained from fifteen donors by aspiration. Lymphocyte proliferation ability, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) secreted by T cells were determined using a monotetrazolium (MTT) assay and sandwich enzyme-linked immunosorbent assay (ELISA), respectively. T-cell subgroups, dendritic cell (DC) subsets, CD4(+)CD25(+) regulatory T cells and the expression of CD28/CD80/CD86 molecules on monocytes, B and T cells were analyzed using flow cytometry.
Results. G-CSF treatment decreased the quantities of IFN-gamma secretion dramatically (p=0.007) and IL-4 moderately (p=0.027), leading to higher ratios of IL-4/IFN-gamma (p=0.004). We confirmed T-cell hyporesponsiveness and lower expression of CD28/CD80/CD86 on monocytes, B and T cells. The absolute values of lymphocytes, T cell subgroups, CD3(+)CD4(-)CD8(-) cells, CD8(+)CD28(-) cells and B cells in bone marrow grafts were similar before and after G-CSF treatment. The number of monocytes per microliter was increased 2.13-fold, while the numbers of CD4(+)CD25(+) regulatory T cells were unchanged. DC2 were preferentially increased.
Interpretation and Conclusions. Our results suggest that bone marrow T-cell hyporesponsiveness could be induced and that the increase of monocytes and DC2 and the downregulation of CD28/CD80/CD86 co-stimulatory signals were produced by in vivo use of G-CSF; this may be related to the preferential increase of monocytes and DC2 and the downregulation of CD28/CD80/CD86 co-stimulatory signals.
|作者单位||Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100044, Peoples R China|
|Jun, HX,Jun, CY,Yu, ZX. In vivo induction of T-cell hyporesponsiveness and alteration of immunological cells of bone marrow grafts using granulocyte colony-stimulating factor[J]. HAEMATOLOGICA,2004,89(12):1517-1524.|
|APA||Jun, HX,Jun, CY,&Yu, ZX.(2004).In vivo induction of T-cell hyporesponsiveness and alteration of immunological cells of bone marrow grafts using granulocyte colony-stimulating factor.HAEMATOLOGICA,89(12),1517-1524.|
|MLA||Jun, HX,et al."In vivo induction of T-cell hyporesponsiveness and alteration of immunological cells of bone marrow grafts using granulocyte colony-stimulating factor".HAEMATOLOGICA 89.12(2004):1517-1524.|