IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa
Wang, Shaoxin1; Pu, Jiang1; Li, Na2; Li, Chuanfeng3; Li, Chao1; Yu, Lan1; Wang, Xiaohui1; Fu, Shanfeng1; Cui, Lihong1
关键词Inflammatory bowel disease CUEDC2 Inflammation Proliferation
刊名DIGESTIVE DISEASES AND SCIENCES
2015-12-01
DOI10.1007/s10620-015-3800-z
60期:12页:3603-3609
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]INFLAMMATORY-BOWEL-DISEASE ; COLON-CANCER ; KAPPA-B ; EXPRESSION ; RECEPTOR
英文摘要

Background CUEDC2, a CUE domain-containing protein, is highly expressed in many tumors, which also may be associated with inflammation.

Aims In this study, we studied whether CUEDC2 plays a role in the progress of inflammatory bowel disease using CUEDC2 knockout (KO) mice and discussed the effects of CUEDC2 on cell proliferation in colonic mucosa.

Methods CUEDC2 KO mice were administered with drinking dextran sodium sulfate (DSS) to establish colitis mice model. At different time points after DSS administration, body weight and stool consistency of mice were graded. Cytokines in colon tissue such as IL-6 were measured by RT-PCR. NF-kappa B and STAT3 signaling pathways in colon tissue were assessed by western blotting. Besides, cell proliferation of intestinal mucosa was analyzed by immunohistochemical staining.

Results CUEDC2 alleviated the colonic inflammation, showing elevated body weight loss, worse diarrhea, and more severe colonic mucosal injury in CUEDC2 KO mice than WT mice. Moreover, pro-inflammatory cytokines such as IL-6, TNF alpha, COX2, and MIP2 were significantly elevated. In CUEDC2 KO mice, the NF-kappa B and STAT3 signaling pathways were increasingly activated in different stages of progression of the colonic inflammation, and the percentage of proliferating cells as indicated by Ki67, CyclinD1, and BrdU in the inflammatory tissues was significantly increased.

Conclusions Our findings demonstrate that CUEDC2 plays an important role in protection from colonic inflammation, primarily by inhibiting the NF-kappa B and STAT3 signaling pathways and preventing excessive proliferation of the inflammatory epithelial cell.

语种英语
WOS记录号WOS:000364563600017
资助机构Navy General Hospital, Beijing, China
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59558
专题北京大学第三临床医学院
作者单位1.Navy Gen Hosp, Dept Digest, Beijing 100048, Peoples R China
2.Navy Gen Hosp, Dept Lab, Beijing 100048, Peoples R China
3.Peking Univ, Hosp 3, Dept Digest, Beijing 100191, Peoples R China
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GB/T 7714
Wang, Shaoxin,Pu, Jiang,Li, Na,et al. CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa[J]. DIGESTIVE DISEASES AND SCIENCES,2015,60(12):3603-3609.
APA Wang, Shaoxin.,Pu, Jiang.,Li, Na.,Li, Chuanfeng.,Li, Chao.,...&Cui, Lihong.(2015).CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa.DIGESTIVE DISEASES AND SCIENCES,60(12),3603-3609.
MLA Wang, Shaoxin,et al."CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa".DIGESTIVE DISEASES AND SCIENCES 60.12(2015):3603-3609.
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