|CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa|
|Wang, Shaoxin1; Pu, Jiang1; Li, Na2; Li, Chuanfeng3; Li, Chao1; Yu, Lan1; Wang, Xiaohui1; Fu, Shanfeng1; Cui, Lihong1|
|关键词||Inflammatory bowel disease CUEDC2 Inflammation Proliferation|
|刊名||DIGESTIVE DISEASES AND SCIENCES|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||INFLAMMATORY-BOWEL-DISEASE ; COLON-CANCER ; KAPPA-B ; EXPRESSION ; RECEPTOR|
Background CUEDC2, a CUE domain-containing protein, is highly expressed in many tumors, which also may be associated with inflammation.
Aims In this study, we studied whether CUEDC2 plays a role in the progress of inflammatory bowel disease using CUEDC2 knockout (KO) mice and discussed the effects of CUEDC2 on cell proliferation in colonic mucosa.
Methods CUEDC2 KO mice were administered with drinking dextran sodium sulfate (DSS) to establish colitis mice model. At different time points after DSS administration, body weight and stool consistency of mice were graded. Cytokines in colon tissue such as IL-6 were measured by RT-PCR. NF-kappa B and STAT3 signaling pathways in colon tissue were assessed by western blotting. Besides, cell proliferation of intestinal mucosa was analyzed by immunohistochemical staining.
Results CUEDC2 alleviated the colonic inflammation, showing elevated body weight loss, worse diarrhea, and more severe colonic mucosal injury in CUEDC2 KO mice than WT mice. Moreover, pro-inflammatory cytokines such as IL-6, TNF alpha, COX2, and MIP2 were significantly elevated. In CUEDC2 KO mice, the NF-kappa B and STAT3 signaling pathways were increasingly activated in different stages of progression of the colonic inflammation, and the percentage of proliferating cells as indicated by Ki67, CyclinD1, and BrdU in the inflammatory tissues was significantly increased.
Conclusions Our findings demonstrate that CUEDC2 plays an important role in protection from colonic inflammation, primarily by inhibiting the NF-kappa B and STAT3 signaling pathways and preventing excessive proliferation of the inflammatory epithelial cell.
|资助机构||Navy General Hospital, Beijing, China|
|作者单位||1.Navy Gen Hosp, Dept Digest, Beijing 100048, Peoples R China|
2.Navy Gen Hosp, Dept Lab, Beijing 100048, Peoples R China
3.Peking Univ, Hosp 3, Dept Digest, Beijing 100191, Peoples R China
|Wang, Shaoxin,Pu, Jiang,Li, Na,et al. CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa[J]. DIGESTIVE DISEASES AND SCIENCES,2015,60(12):3603-3609.|
|APA||Wang, Shaoxin.,Pu, Jiang.,Li, Na.,Li, Chuanfeng.,Li, Chao.,...&Cui, Lihong.(2015).CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa.DIGESTIVE DISEASES AND SCIENCES,60(12),3603-3609.|
|MLA||Wang, Shaoxin,et al."CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa".DIGESTIVE DISEASES AND SCIENCES 60.12(2015):3603-3609.|