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学科主题: 临床医学
题名:
CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa
作者: Wang, Shaoxin1; Pu, Jiang1; Li, Na2; Li, Chuanfeng3; Li, Chao1; Yu, Lan1; Wang, Xiaohui1; Fu, Shanfeng1; Cui, Lihong1
关键词: Inflammatory bowel disease ; CUEDC2 ; Inflammation ; Proliferation
刊名: DIGESTIVE DISEASES AND SCIENCES
发表日期: 2015-12-01
DOI: 10.1007/s10620-015-3800-z
卷: 60, 期:12, 页:3603-3609
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; COLON-CANCER ; KAPPA-B ; EXPRESSION ; RECEPTOR
英文摘要:

Background CUEDC2, a CUE domain-containing protein, is highly expressed in many tumors, which also may be associated with inflammation.

Aims In this study, we studied whether CUEDC2 plays a role in the progress of inflammatory bowel disease using CUEDC2 knockout (KO) mice and discussed the effects of CUEDC2 on cell proliferation in colonic mucosa.

Methods CUEDC2 KO mice were administered with drinking dextran sodium sulfate (DSS) to establish colitis mice model. At different time points after DSS administration, body weight and stool consistency of mice were graded. Cytokines in colon tissue such as IL-6 were measured by RT-PCR. NF-kappa B and STAT3 signaling pathways in colon tissue were assessed by western blotting. Besides, cell proliferation of intestinal mucosa was analyzed by immunohistochemical staining.

Results CUEDC2 alleviated the colonic inflammation, showing elevated body weight loss, worse diarrhea, and more severe colonic mucosal injury in CUEDC2 KO mice than WT mice. Moreover, pro-inflammatory cytokines such as IL-6, TNF alpha, COX2, and MIP2 were significantly elevated. In CUEDC2 KO mice, the NF-kappa B and STAT3 signaling pathways were increasingly activated in different stages of progression of the colonic inflammation, and the percentage of proliferating cells as indicated by Ki67, CyclinD1, and BrdU in the inflammatory tissues was significantly increased.

Conclusions Our findings demonstrate that CUEDC2 plays an important role in protection from colonic inflammation, primarily by inhibiting the NF-kappa B and STAT3 signaling pathways and preventing excessive proliferation of the inflammatory epithelial cell.

语种: 英语
项目资助者: Navy General Hospital, Beijing, China
WOS记录号: WOS:000364563600017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59558
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Navy Gen Hosp, Dept Digest, Beijing 100048, Peoples R China
2.Navy Gen Hosp, Dept Lab, Beijing 100048, Peoples R China
3.Peking Univ, Hosp 3, Dept Digest, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Shaoxin,Pu, Jiang,Li, Na,et al. CUEDC2 Protects Against Experimental Colitis and Suppresses Excessive Proliferation of Intestinal Mucosa[J]. DIGESTIVE DISEASES AND SCIENCES,2015,60(12):3603-3609.
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