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Gene expression profiles in response to the activation of adrenoceptors in A7R5 aortic smooth muscle cells
Wang, YY; Hou, R; Li, P; Li, JL; Yan, J; Yin, F; Han, C; Zhang, YY
关键词adrenoceptors DNA microarray gene expression profile proliferation vascular smooth muscle cells
刊名CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
2004-09-01
31期:9页:602-607
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Physiology
研究领域[WOS]Pharmacology & Pharmacy ; Physiology
关键词[WOS]RAT AORTA ; STIMULATION ; PATTERNS ; SUBTYPES
英文摘要

1. Vascular adrenoceptors play an important role in vascular physiology and pathophysiology, such as hypertension, atherosclerosis and restenosis after angioplasty. To define the changes in the ene expression in vascular smooth muscle cells in response to the activation of alpha(1)- or beta-adrenoceptors, a DNA microarray was used.

2. First, the existence of alpha(1)- and beta-adrenoceptors in A7r5 aortic smooth muscle cells was confirmed by radioligand binding. Then, the inhibitory effects of phenylephrine (an alpha(1)-adrenoceptor agonist) and isoproterenol (a beta-adrenoceptor agonist) on the proliferation of A7r5 cells were determined by [H-3]-thymidine incorporation.

3. The A7r5 cells were treated with 10 mumol/L phenylephrine or 1 mumol/L isoproterenol for 24 h and changes in gene expression were detected with the DNA microarray. Only 14 and 20 genes were identified after treatment of cells with phenylephrine and isoproterenol, respectively, and most genes displayed decreased expression. The changed genes could be grouped into five major functional categories: cell signalling/ communication, cell structure/motility, cell/organism defence, gene/protein expression and metabolism. The gene expression profile in response to the activation of alpha(1)-adrenoceptors was very different from that following activation of beta-adrenoceptors. Interestingly, many phenylephrine-responsive genes were associated with metabolism, whereas many isoproterenol-responsive genes encoded cell signalling and structure proteins. This means that adrenoceptors may modulate multiple aspects of biological function in vascular smooth muscle cells.

4. Collectively, the activation of both alpha(1)-adrenoceptors (with phenylephrine) and beta-adrenoceptors (with isoproterenol) inhibited the proliferation of A7r5 cells, but microarray data revealed that the mechanisms may be different: the activation of alpha(1)-adrenoceptors could induce the expression of metabolic genes, resulting in the inhibition of proliferation, whereas activation of beta-adrenoceptors altered the expression of genes that encoded cell signalling and structure proteins to inhibit cell proliferation.

语种英语
WOS记录号WOS:000224248600007
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59565
专题北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
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GB/T 7714
Wang, YY,Hou, R,Li, P,et al. Gene expression profiles in response to the activation of adrenoceptors in A7R5 aortic smooth muscle cells[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2004,31(9):602-607.
APA Wang, YY.,Hou, R.,Li, P.,Li, JL.,Yan, J.,...&Zhang, YY.(2004).Gene expression profiles in response to the activation of adrenoceptors in A7R5 aortic smooth muscle cells.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,31(9),602-607.
MLA Wang, YY,et al."Gene expression profiles in response to the activation of adrenoceptors in A7R5 aortic smooth muscle cells".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 31.9(2004):602-607.
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