IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma
Dong, Ling1,2; Ge, Xi-Yuan1,2,3,4; Wang, Yi-Xiang1,2,3,4; Yang, Lin-Qian3,4; Li, Sheng-Lin1,2,3,4; Yu, Guang-Yan5,6; Gao, Yan7,8; Fu, Jia3,4
关键词Adenoid cystic carcinoma Pulmonary metastasis Transforming growth factor-beta 1 Epithelial-mesenchymal transition Gene therapy
刊名ORAL ONCOLOGY
2013-11-01
DOI10.1016/j.oraloncology.2013.07.012
49期:11页:1051-1058
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Dentistry, Oral Surgery & Medicine
研究领域[WOS]Oncology ; Dentistry, Oral Surgery & Medicine
关键词[WOS]CANCER PROGRESSION ; E-CADHERIN ; EXPRESSION ; INVASION ; TUMOR ; CATENIN ; SUPPRESSOR ; EXPERIENCE ; RECEPTORS ; PATHWAYS
英文摘要

Objectives: Adenoid cystic carcinoma (ACC) is one of the most common malignancies of salivary glands, characterized by poor prognosis, particularly due to pulmonary metastasis. We previously reported that transforming growth factor (TGF)-beta 1 promoted ACC cell migration and invasion via the Smad pathway in vitro. The aim of this study was to establish the underlying mechanisms.

Materials and methods: TGF-beta 1, phospho-Smad2 and beta-catenin expression in ACC tissues derived from patients was evaluated by immunohistochemistry. The role of TGF- beta 1 on the invasive capacity of ACC cells was determined by transwell assays in SACC-83 cells transfected with TGF-beta 1 and TGF-beta type II dominant-negative receptor (T beta RIIDN) plasmids or silenced by TGF-beta 1 siRNA. Expression of the epithelial-mesenchymal transition (EMT) markers, beta-catenin, E-cadherin and Nectin-1, was determined by real-time PCR and immunochemistry. In vivo investigations were performed by inoculating nude mice with the transfected ACC cells and examining metastasis in bilateral lung tissues by immunohistochemistry.

Results: Overexpression of TGF-beta 1 and phospho-Smad2, and reduced expression of membrane beta-catenin, were closely associated with lung metastasis in ACC. Furthermore, the EMT markers were downregulated. In vitro, cells transfected with TGF-beta 1 exhibited altered morphology and increased invasive capacity compared to T beta RIIDN-transfected cells or TGF-beta 1 siRNA silenced cells. In vivo, mice inoculated with TGF-beta 1 transfected ACC cells exhibited more metastases than other cells.

Conclusion: TGF-beta 1, phospho-Smad2 and beta-catenin were significantly correlated with ACC metastasis. Blockade of TGF-beta signaling by T beta RIIDN or siRNA may offer potential gene therapies against pulmonary metastasis in patients with ACC. (c) 2013 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000325461200003
项目编号30228028 ; 30572050 ; 39470754 ; 7062069
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59567
专题北京大学口腔医学院
北京大学口腔医学院_外科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch Stomatol, Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Hosp Stomatol, Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100081, Peoples R China
4.Peking Univ, Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
5.Peking Univ, Sch Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
6.Peking Univ, Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
7.Peking Univ, Sch Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
8.Peking Univ, Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
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GB/T 7714
Dong, Ling,Ge, Xi-Yuan,Wang, Yi-Xiang,et al. Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma[J]. ORAL ONCOLOGY,2013,49(11):1051-1058.
APA Dong, Ling.,Ge, Xi-Yuan.,Wang, Yi-Xiang.,Yang, Lin-Qian.,Li, Sheng-Lin.,...&Fu, Jia.(2013).Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma.ORAL ONCOLOGY,49(11),1051-1058.
MLA Dong, Ling,et al."Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma".ORAL ONCOLOGY 49.11(2013):1051-1058.
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