北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 期刊论文
学科主题: 口腔医学
题名:
Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma
作者: Dong, Ling1,2; Ge, Xi-Yuan1,2,3,4; Wang, Yi-Xiang1,2,3,4; Yang, Lin-Qian3,4; Li, Sheng-Lin1,2,3,4; Yu, Guang-Yan5,6; Gao, Yan7,8; Fu, Jia3,4
关键词: Adenoid cystic carcinoma ; Pulmonary metastasis ; Transforming growth factor-beta 1 ; Epithelial-mesenchymal transition ; Gene therapy
刊名: ORAL ONCOLOGY
发表日期: 2013-11-01
DOI: 10.1016/j.oraloncology.2013.07.012
卷: 49, 期:11, 页:1051-1058
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
关键词[WOS]: CANCER PROGRESSION ; E-CADHERIN ; EXPRESSION ; INVASION ; TUMOR ; CATENIN ; SUPPRESSOR ; EXPERIENCE ; RECEPTORS ; PATHWAYS
英文摘要:

Objectives: Adenoid cystic carcinoma (ACC) is one of the most common malignancies of salivary glands, characterized by poor prognosis, particularly due to pulmonary metastasis. We previously reported that transforming growth factor (TGF)-beta 1 promoted ACC cell migration and invasion via the Smad pathway in vitro. The aim of this study was to establish the underlying mechanisms.

Materials and methods: TGF-beta 1, phospho-Smad2 and beta-catenin expression in ACC tissues derived from patients was evaluated by immunohistochemistry. The role of TGF- beta 1 on the invasive capacity of ACC cells was determined by transwell assays in SACC-83 cells transfected with TGF-beta 1 and TGF-beta type II dominant-negative receptor (T beta RIIDN) plasmids or silenced by TGF-beta 1 siRNA. Expression of the epithelial-mesenchymal transition (EMT) markers, beta-catenin, E-cadherin and Nectin-1, was determined by real-time PCR and immunochemistry. In vivo investigations were performed by inoculating nude mice with the transfected ACC cells and examining metastasis in bilateral lung tissues by immunohistochemistry.

Results: Overexpression of TGF-beta 1 and phospho-Smad2, and reduced expression of membrane beta-catenin, were closely associated with lung metastasis in ACC. Furthermore, the EMT markers were downregulated. In vitro, cells transfected with TGF-beta 1 exhibited altered morphology and increased invasive capacity compared to T beta RIIDN-transfected cells or TGF-beta 1 siRNA silenced cells. In vivo, mice inoculated with TGF-beta 1 transfected ACC cells exhibited more metastases than other cells.

Conclusion: TGF-beta 1, phospho-Smad2 and beta-catenin were significantly correlated with ACC metastasis. Blockade of TGF-beta signaling by T beta RIIDN or siRNA may offer potential gene therapies against pulmonary metastasis in patients with ACC. (c) 2013 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30228028 ; 30572050 ; 39470754 ; 7062069
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000325461200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59567
Appears in Collections:北京大学口腔医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Stomatol, Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Hosp Stomatol, Lab Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100081, Peoples R China
4.Peking Univ, Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
5.Peking Univ, Sch Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
6.Peking Univ, Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
7.Peking Univ, Sch Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
8.Peking Univ, Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China

Recommended Citation:
Dong, Ling,Ge, Xi-Yuan,Wang, Yi-Xiang,et al. Transforming growth factor-beta and epithelial-mesenchymal transition are associated with pulmonary metastasis in adenoid cystic carcinoma[J]. ORAL ONCOLOGY,2013,49(11):1051-1058.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dong, Ling]'s Articles
[Ge, Xi-Yuan]'s Articles
[Wang, Yi-Xiang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dong, Ling]‘s Articles
[Ge, Xi-Yuan]‘s Articles
[Wang, Yi-Xiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace