|Matrix metalloproteinase-8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients|
|Chen, HY; Cox, SW; Eley, BM; Mantyla, P; Ronka, H; Sorsa, T|
|关键词||gingival crevicular fluid periodontitis matrix metalloproteinase-8 collagenase elastase|
|刊名||JOURNAL OF CLINICAL PERIODONTOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||CATHEPSIN-B/L-LIKE ; IV-LIKE ACTIVITIES ; COLLAGENASE ACTIVITY ; SULCULAR FLUID ; TRYPTASE-LIKE ; TRYPSIN-LIKE ; NEUTROPHIL ; INHIBITORS|
Aim: To make an initial assessment of the periodontal diagnostic potential of immunoreactive matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF) by comparison with elastase activity which has previously been associated with disease severity and progression.
Methods: GCF was collected from molar and premolar sites of 16 chronic adult periodontitis patients before treatment and 13 of this group 2 weeks after scaling and root planing. Samples were analysed for MMP-8 by immunofluorometric assay and for elastase activity with a fluorogenic substrate.
Results: Mean patient clinical parameters and GCF enzyme totals both decreased significantly after treatment. Total MMP-8 levels and elastase activities generally correlated significantly with gingival and bleeding indices. For GCF concentrations, only MMP-8 showed a significant fall after treatment, and some significant correlations with clinical parameters. Amounts of the 2 enzymes correlated significantly with each other.
Conclusions: Similarities between MMP-8 and elastase probably reflect the fact that both enzymes are associated mainly with neutrophils: MMP-8 levels may have fallen more after treatment because the assay, unlike that for elastase, would most likely not have detected much enzyme bound to alpha(2)-macroglobulin. The immunoassay for MMP-8 is more specific and convenient than functional collagenase assays, and might be suitable for monitoring the periodontal condition.
|作者单位||1.Univ Helsinki, Inst Dent, Dept Periodontol, Helsinki, Finland|
2.Oy Ale, Medix Biochem, Kauniainen, Finland
3.Guys Kings & St Thomas Dent Inst, Dept Periodontol, London SE5 9RW, England
4.Beijing Med Univ, Clin Sch 3, Dept Stomatol, Beijing 100083, Peoples R China
|Chen, HY,Cox, SW,Eley, BM,et al. Matrix metalloproteinase-8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients[J]. JOURNAL OF CLINICAL PERIODONTOLOGY,2000,27(5):366-369.|
|APA||Chen, HY,Cox, SW,Eley, BM,Mantyla, P,Ronka, H,&Sorsa, T.(2000).Matrix metalloproteinase-8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients.JOURNAL OF CLINICAL PERIODONTOLOGY,27(5),366-369.|
|MLA||Chen, HY,et al."Matrix metalloproteinase-8 levels and elastase activities in gingival crevicular fluid from chronic adult periodontitis patients".JOURNAL OF CLINICAL PERIODONTOLOGY 27.5(2000):366-369.|