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学科主题: 药学
题名:
Quality Assessment of Commercial Magnoliae Officinalis Cortex by 1H-NMR-based Metabolomics and HPLC Methods
作者: Jiang, Yong1; Vaysse, Julie4,5; Gilard, Veronique4,5; Balayssac, Stephane4,5; Dejean, Sebastien3; Malet-Martino, Myriam4,5; David, Bruno2; Fiorini, Christel2; Barbin, Yves2
关键词: 1H NMR ; HPLC ; metabolomics ; quality control ; Magnoliae officinalis Cortex
刊名: PHYTOCHEMICAL ANALYSIS
发表日期: 2012-07-01
DOI: 10.1002/pca.1369
卷: 23, 期:4, 页:387-395
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Plant Sciences ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
关键词[WOS]: NUCLEAR-MAGNETIC-RESONANCE ; PRINCIPAL COMPONENT ANALYSIS ; MULTIVARIATE DATA-ANALYSIS ; H-1-NMR SPECTROSCOPY ; ELECTROCHEMICAL DETECTION ; GINSENG ROOTS ; NMR ; CLASSIFICATION ; PRODUCTS ; HONOKIOL
英文摘要:

Introduction The quality control of Magnoliae officinalis Cortex, a commonly used traditional Chinese medicine, is currently based on the assay of the two active compounds, honokiol and magnolol, by TLC or HPLC. Objective To compare 1H-NMR-based metabolomics with the HPLC method for controlling the quality of Magnoliae officinalis Cortex. To identify the metabolites contributing to the differences between the samples and to discriminate different medicinal parts and geographic origins of these samples by 1H-NMR-based metabolomics. Methodology 1H-NMR and several multivariate analysis techniques were applied to analyse the extracts of 18 batches of Magnoliae officinalis Cortex commercial samples, and the contents of honokiol and magnolol in these samples were determined by HPLC. The correlation analysis between the data from 1H-NMR and HPLC was performed with the mixOmics software based on an unsupervised method. Results Honokiol and magnolol were the main compounds responsible for the discrimination of samples from different batches, thus proving that the choice of these two compounds as markers for quality assessment by HPLC is relevant. The two sources of Magnoliae officinalis Cortex recorded in the Chinese Pharmacopoeia, Magnolia officinalis and Magnolia officinalis var. biloba, could be differentiated from 1H-NMR data, but the pattern recognition analysis by PLS-DA was unsuccessful in discriminating samples from various geographical origins. Conclusion The combination of 1H-NMR that gives a comprehensive profile of the metabolites and HPLC that targets two biomarkers is an efficient means for a better quality control of Magnoliae officinalis Cortex. Copyright (C) 2011 John Wiley & Sons, Ltd.

语种: 英语
项目资助者: Fondation Franco-Chinoise pour la Science et ses Applications (FFCSA) ; China Scholarship Council (CSC) ; Conseil Regional MidiPyrenees and Laboratoires Pierre Fabre
WOS记录号: WOS:000305571500014
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59587
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Inst Rech Pierre Fabre, Pole Actifs Vegetaux, Branche Sourcing R&D & Bot, F-31035 Toulouse 1, France
3.Univ Toulouse, Inst Math Toulouse, UMR 5219, F-31062 Toulouse 9, France
4.UPS, F-31062 Toulouse 9, France
5.Lab Synthese & Physicochim Mol Interet Biol SPCMI, Grp RMN Biomed, F-31062 Toulouse 9, France

Recommended Citation:
Jiang, Yong,Vaysse, Julie,Gilard, Veronique,et al. Quality Assessment of Commercial Magnoliae Officinalis Cortex by 1H-NMR-based Metabolomics and HPLC Methods[J]. PHYTOCHEMICAL ANALYSIS,2012,23(4):387-395.
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