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学科主题: 临床医学
题名:
Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro
作者: Guo, Lili1; Yu, Wenzhen1; Li, Xiaoxin1; Zhao, Gang3; He, Peiying2
关键词: Endothelial cells ; Integrin-linked kinase ; Small interfering RNA
刊名: OPHTHALMIC RESEARCH
发表日期: 2009
DOI: 10.1159/000232971
卷: 42, 期:4, 页:213-220
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: GLYCOGEN-SYNTHASE KINASE ; PROTEIN-KINASE ; TUMOR ANGIOGENESIS ; GROWTH-FACTOR ; CANCER CELLS ; ILK ; INVASION ; TRANSCRIPTION ; EXPRESSION ; SURVIVAL
英文摘要:

Purpose: To investigate the role of integrin-linked kinase (ILK) in endothelial cell migration, proliferation and tube formation in vitro. Materials and Methods: Cultured RF/6A cells were knocked down for ILK using small interfering RNA (siRNA). Cellular ILK expression was quantified by real-time quantitative PCR and Western blot analysis. Cell migration was measured by cell counting in modified Boyden chambers, while microfilament dynamics were assessed by immunofluorescence analysis. Cell cycling was determined using a FACS Calibur flow cytometer, and the expression of cyclin D 1 was also measured by the Western blot assay. The secretion of vascular endothelial growth factor (VEGF) in culture medium samples was assessed by ELISA, and capillary/tubelike network formation assays were performed using matrigel. Results: Both ILK mRNA and protein levels were virtually undetectable after transfection with ILK siRNA. In addition, there was a significant accumulation of cells in the G(0)-G(1) phase and a marked decrease in cell numbers in the S phase in ILK-specific siRNA-transfected cells, and the expression of cyclin D-1 was decreased after transfection. The knockdown of ILK significantly inhibited cell migration ability by disturbing F actin assembly, and VEGF secretion in conditioned medium was also reduced by 33%. Furthermore, treatment with ILK siRNA suppressed tube formation in RF/6A cells and significantly reduced the overall length of the tubes. Conclusions: Knockdown of ILK with siRNA effectively inhibits endothelial cell migration, proliferation and tube formation in vitro. Copyright (C) 2009 S. Karger AG, Basel

语种: 英语
所属项目编号: 7092110
项目资助者: Beijing Natural Science Foundation
WOS记录号: WOS:000272520900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59599
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
3.Beijing Hosp, Minist Hlth, Dept Gen Surg, Beijing, Peoples R China

Recommended Citation:
Guo, Lili,Yu, Wenzhen,Li, Xiaoxin,et al. Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro[J]. OPHTHALMIC RESEARCH,2009,42(4):213-220.
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