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Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro
Guo, Lili1; Yu, Wenzhen1; Li, Xiaoxin1; Zhao, Gang3; He, Peiying2
关键词Endothelial cells Integrin-linked kinase Small interfering RNA
刊名OPHTHALMIC RESEARCH
2009
DOI10.1159/000232971
42期:4页:213-220
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]GLYCOGEN-SYNTHASE KINASE ; PROTEIN-KINASE ; TUMOR ANGIOGENESIS ; GROWTH-FACTOR ; CANCER CELLS ; ILK ; INVASION ; TRANSCRIPTION ; EXPRESSION ; SURVIVAL
英文摘要

Purpose: To investigate the role of integrin-linked kinase (ILK) in endothelial cell migration, proliferation and tube formation in vitro. Materials and Methods: Cultured RF/6A cells were knocked down for ILK using small interfering RNA (siRNA). Cellular ILK expression was quantified by real-time quantitative PCR and Western blot analysis. Cell migration was measured by cell counting in modified Boyden chambers, while microfilament dynamics were assessed by immunofluorescence analysis. Cell cycling was determined using a FACS Calibur flow cytometer, and the expression of cyclin D 1 was also measured by the Western blot assay. The secretion of vascular endothelial growth factor (VEGF) in culture medium samples was assessed by ELISA, and capillary/tubelike network formation assays were performed using matrigel. Results: Both ILK mRNA and protein levels were virtually undetectable after transfection with ILK siRNA. In addition, there was a significant accumulation of cells in the G(0)-G(1) phase and a marked decrease in cell numbers in the S phase in ILK-specific siRNA-transfected cells, and the expression of cyclin D-1 was decreased after transfection. The knockdown of ILK significantly inhibited cell migration ability by disturbing F actin assembly, and VEGF secretion in conditioned medium was also reduced by 33%. Furthermore, treatment with ILK siRNA suppressed tube formation in RF/6A cells and significantly reduced the overall length of the tubes. Conclusions: Knockdown of ILK with siRNA effectively inhibits endothelial cell migration, proliferation and tube formation in vitro. Copyright (C) 2009 S. Karger AG, Basel

语种英语
WOS记录号WOS:000272520900006
项目编号7092110
资助机构Beijing Natural Science Foundation
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59599
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
3.Beijing Hosp, Minist Hlth, Dept Gen Surg, Beijing, Peoples R China
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GB/T 7714
Guo, Lili,Yu, Wenzhen,Li, Xiaoxin,et al. Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro[J]. OPHTHALMIC RESEARCH,2009,42(4):213-220.
APA Guo, Lili,Yu, Wenzhen,Li, Xiaoxin,Zhao, Gang,&He, Peiying.(2009).Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro.OPHTHALMIC RESEARCH,42(4),213-220.
MLA Guo, Lili,et al."Targeting of Integrin-Linked Kinase with a Small Interfering RNA Inhibits Endothelial Cell Migration, Proliferation and Tube Formation in vitro".OPHTHALMIC RESEARCH 42.4(2009):213-220.
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