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学科主题: 基础医学
题名:
Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes
作者: Liu, X-X1; Liu, K. -Y.2; Li, P.1; Han, S.1; Peng, X. -D.3; Shen, L.1
关键词: Adiponectin ; Type 2 diabetes ; Pancreas ; Vascular endothelial cells ; High-fat diet
刊名: DIABETES & METABOLISM
发表日期: 2014-11-01
DOI: 10.1016/j.diabet.2014.05.004
卷: 40, 期:5, 页:363-372
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
关键词[WOS]: INSULIN GENE-EXPRESSION ; DOWN-REGULATION ; GAMMA ACTIVITY ; CELL ; BETA ; RECEPTOR ; DYSFUNCTION ; RESISTANCE ; SECRETION ; GLUCOSE
英文摘要:

Aim. - Adiponectin levels in skeletal muscle and adipose tissue have been reported to be involved in insulin resistance in rats fed with a high-fat diet (HFD). Our objective was to explore whether adiponectin is also expressed in the pancreas and what its potential role is during the development of type 2 diabetes (T2D) in outbred CD-1 mice.

Methods. - Male 4-week-old outbred CD-1 mice were fed an HFD to induce a polygenic model of human T2D. Adiponectin expression was examined in mouse pancreas by quantitative real-time polymerase chain reaction (qPCR), western blots and immunofluorescence analyses. Human umbilical vein endothelium cells (HUVECs) were transfected with an adiponectin-expressing lentivirus to determine the effect of adiponectin on angiogenic function in vitro.

Results. - Feeding mice an HFD for 9 weeks resulted in constant hyperglycaemia, obesity, impaired glucose tolerance and insulin resistance. Additional hyperinsulinaemia emerged in mice fed an HFD for 18 weeks. Interestingly, aberrant expression of adiponectin was detectable in the pancreatic vascular endothelial cells (VECs) of mice fed with an HFD, but not in mice fed with regular chow (RC). Expression levels of pancreatic adiponectin varied during the development of T2D. This extraordinary expression of adiponectin in pancreatic VECs played a role in protecting endothelial function against potential damage by HFD. Our in vitro study has demonstrated that adiponectin promotes angiogenic function.

Conclusion. - These results reveal for the first time that adiponectin is expressed in pancreatic VECs of HFD-fed mice during the development of T2D as a protective adaptation in response to the HFD. (C) 2014 Elsevier Masson SAS. All rights reserved.

语种: 英语
所属项目编号: 2012AA020505 ; 81272790
项目资助者: National High-tech R&amp ; D Program of China (863 Program) ; National Natural Science Foundation of China
WOS记录号: WOS:000346231100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59607
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Univ Illinois, Dept Biochem & Mol Genet, Chicago, IL 60607 USA

Recommended Citation:
Liu, X-X,Liu, K. -Y.,Li, P.,et al. Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes[J]. DIABETES & METABOLISM,2014,40(5):363-372.
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