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学科主题: 基础医学
题名:
Efficient Induction of Pluripotent Stem Cells from Menstrual Blood
作者: Li, Yang1,2; Li, Xiaoni3; Zhao, Hongxi4; Feng, Ruopeng1,2,5; Zhang, Xiaoyan1,2; Tai, Dapeng6; An, Guangyu7; Wen, Jinhua1,2; Tan, Jichun3
刊名: STEM CELLS AND DEVELOPMENT
发表日期: 2013-04-01
DOI: 10.1089/scd.2012.0428
卷: 22, 期:7, 页:1147-1158
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: HUMAN SOMATIC-CELLS ; HUMAN FIBROBLASTS ; DEFINED FACTORS ; GENERATION ; DIFFERENTIATION ; OCT4
英文摘要:

The technology to reprogram human somatic cells back to pluripotency allows the production of patient-specific induced pluripotent stem cells (iPSCs) and holds a great promise for regenerative medicine. Choosing the most suitable cell type for induction and reducing the risk of viral transgene activation, especially oncogene activation, are important for iPSC research. To date, human dermal fibroblasts (HDFs) are the most frequent cell source used for iPSC generation, but they have several limitations. An invasive skin biopsy must be performed to obtain HDFs, and HDFs must be cultured for a prolonged period before they can be used for experiments. Thus, in an effort to develop a suitable source for iPSC studies to avoid the limitations mentioned above, we have here identified stromal cells derived from menstrual blood (MenSCs) as suitable candidates. In the present study, we found that MenSCs can be reprogrammed to pluripotent status by doxycycline-inducible lentiviral transduction of OCT4, SOX2, and KLF4. Additionally, we found that MenSCs have a significantly higher reprogramming efficiency than HDFs. The combination of OCT4 and SOX2 is sufficient to reprogram MenSCs into iPSCs without the use of c-MYC or KLF4. The resulting MenSC-iPSCs showed the same characteristics as human embryonic stem cells with regard to morphology, pluripotent markers, gene expression, and the epigenetic status of pluripotent-cell-specific genes. These cells were able to differentiate into various cell types of all 3 germ layers both in vitro and in vivo. Therefore, MenSCs may be a preferred candidate for generation of iPSCs.

语种: 英语
所属项目编号: 2011CB966200 ; 31000653 ; 31101060 ; 31171417 ; 5112019 ; 20100001120044 ; 11220129
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China (NSFC) ; Beijing Natural Science Foundation ; Ph.D. Programs Foundation of Ministry of Education of China ; General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China
WOS记录号: WOS:000316946200014
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59619
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Stem Cell Res Ctr, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing 100871, Peoples R China
3.China Med Univ, Shengjing Hosp, Shenyang 110004, Peoples R China
4.Fourth Mil Med Univ, Tangdu Hosp, Dept Gynecol & Obstet, Xian 710032, Peoples R China
5.Baotou Med Coll, Baotou, Peoples R China
6.Inner Mongolia Univ, Coll Life Sci, Lab Anim Res Ctr, Hohhot, Peoples R China
7.Capital Med Univ, Beijing Chaoyang Hosp, Beijing, Peoples R China

Recommended Citation:
Li, Yang,Li, Xiaoni,Zhao, Hongxi,et al. Efficient Induction of Pluripotent Stem Cells from Menstrual Blood[J]. STEM CELLS AND DEVELOPMENT,2013,22(7):1147-1158.
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