|Alleviation of hypoxic pulmonary vascular structural remodeling by L-arginine|
|Qi, JG; Du, JB; Wang, L; Zhao, B; Tang, CS|
|关键词||L-arginine endothelin pulmonary artery hypoxia nitric oxide|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||SMOOTH-MUSCLE CELLS ; NITRIC-OXIDE ; INTRAPULMONARY ARTERIES ; HYPERTENSION ; RATS ; PROLIFERATION ; NEWBORN|
Objective To explore the effect of L-arginine on hypoxic pulmonary vascular structural remodeling and its possible mechanisms.
Methods Eighteen Wistar rats were randomly divided into three groups: the hypoxia group, the hypoxia with L-arginine group and the control group. Pulmonary artery mean pressure was evaluated with right cardiac catheterization. Pulmonary vascular structural changes were also observed. Plasma concentration of nitric oxide (NO) was measured via spectrophotometry, and endothelin-1 (ET-1) mRNA expression in pulmonary artery endothelial cells was detected using in situ hybridization.
Results The pulmonary artery mean pressure was significantly high in hypoxic rats than in normal controls (20.33 +/- 2.18 mm Hg vs 15.38 +/- 1.05 mm Hg, P < 0. 05). Microstructural and ultrastructural analysis revealed the development of hypoxic pulmonary vascular structural remodeling in the hypoxic rats. Meanwhile, the plasma NO concentration was markedly lower in the hypoxic rats than in controls ( P < 0. 05). The expression signals of ET-1 mRNA by pulmonary artery endothelial cells of hypoxic rats strengthened obviously. L-arginine ameliorated pulmonary hypertension (16.73 +/- 1.35 mm Hg vs 20.33 +/- 2.18 mm Hg, P < 0.05) as well as pulmonary vascular structural remodeling in the hypoxic rats in association with an increase in plasma NO concentration ( P < 0. 05) and inhibited ET-1 mRNA expression by the endothelial cells of pulmonary arteries.
Conclusion L-arginine might play an important role in the regulation of hypoxic pulmonary vascular structural remodeling and hypoxic pulmonary hypertension. The mechanism is probably related to promoting NO production and, as a result, inhibiting ET-1 mRNA expression by pulmonary artery endothelial cells in hypoxic rats.
|作者单位||1.Beijing Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China|
2.Beijing Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
3.Beijing Univ, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Beijing Ji Shui Tan Hosp, Dept Internal Med, Beijing 100034, Peoples R China
|Qi, JG,Du, JB,Wang, L,et al. Alleviation of hypoxic pulmonary vascular structural remodeling by L-arginine[J]. CHINESE MEDICAL JOURNAL,2001,114(9):933-936.|
|APA||Qi, JG,Du, JB,Wang, L,Zhao, B,&Tang, CS.(2001).Alleviation of hypoxic pulmonary vascular structural remodeling by L-arginine.CHINESE MEDICAL JOURNAL,114(9),933-936.|
|MLA||Qi, JG,et al."Alleviation of hypoxic pulmonary vascular structural remodeling by L-arginine".CHINESE MEDICAL JOURNAL 114.9(2001):933-936.|