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学科主题: 临床医学
题名:
Up-regulation of mitochondrial antioxidation signals in ovarian cancer cells with aggressive biologic behavior
作者: Wang, Yue1; Dong, Li2; Cui, Heng1; Shen, Dan-hua3; Wang, Ying3; Chang, Xiao-hong1; Fu, Tian-yun1; Ye, Xue1; Yao, Yuan-yang1
关键词: Ovarian carcinoma ; Mitochondria ; Invasion ; Proteomic ; Superoxide dismutase 2 (SOD2)
刊名: JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
发表日期: 2011-05-01
DOI: 10.1631/jzus.B1000192
卷: 12, 期:5, 页:346-356
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]: SUPEROXIDE-DISMUTASE EXPRESSION ; CARCINOMA ; SOD2 ; ASSOCIATION ; PROGRESSION ; METASTASIS ; GENE ; GENERATION ; MUTATIONS ; DEATH
英文摘要:

Recently, a high frequency of mutations in mitochondrial DNA (mtDNA) has been detected in ovarian cancer. To explore the alterations of proteins in mitochondria in ovarian cancer, a pair of human ovarian carcinoma cell lines (SKOV3/SKOV3.ip1) with different metastatic potentials was examined.

Cancer cells SKOV3.ip1 were derived from the ascitic tumor cells of nude mice bearing a tumor of ovarian cancer cells SKOV3. SKOV3.ip1 exhibited a higher degree of migration potential than its paired cell line SKOV3. The proteins in the mitochondria of these two cells were isolated and separated by 2-D gel electrophoresis. The differently expressed proteins were extracted and identified using matrix assisted laser desorption ionisation/time-of-flight/time-of-flight (MALDI-TOF/TOF), and finally a selected protein candidate was further investigated by immunohistochemistry (IHC) method in nude mice bearing tumor tissues of these two cells.

A total of 35 spots with different expressions were identified between the two cells using 2D-polyacrylamide gel electrophoresis (PAGE) approach. Among them, 17 spots were detected only in either SKOV3 or SKOV3.ip1 cells. Eighteen spots expressed different levels, with as much as a three-fold difference between the two cells. Twenty spots were analyzed using MALDI-TOF/TOF, and 11 of them were identified successfully; four were known to be located in mitochondria, including superoxide dismutase 2 (SOD2), fumarate hydratase (FH), mitochondrial ribosomal protein L38 (MRPL38), and mRNA turnover 4 homolog (MRTO4). An increased staining of SOD2 was observed in SKOV3.ip1 over that of SKOV3 in IHC analysis.

Our results indicate that the enhanced antioxidation and metabolic potentials of ovarian cancer cells might contribute to their aggressive and metastatic behaviors. The underlying mechanism warrants further study.

语种: 英语
WOS记录号: WOS:000290277400002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59629
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Peking Univ, Beijing Jishuitan Hosp, Dept Obstet & Gynecol, Beijing 100035, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Pathol, Beijing 100044, Peoples R China

Recommended Citation:
Wang, Yue,Dong, Li,Cui, Heng,et al. Up-regulation of mitochondrial antioxidation signals in ovarian cancer cells with aggressive biologic behavior[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2011,12(5):346-356.
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