IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs
Jiang, Jingjing1,2,3; Huang, Lvzhen1,2; Yu, Wenzhen1,2; Wu, Xi1,2; Zhou, Peng1,2,4; Li, Xiaoxin1,2
刊名PLOS ONE
2012-10-08
DOI10.1371/journal.pone.0046115
7期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]POLYPOIDAL CHOROIDAL VASCULOPATHY ; SERINE-PROTEASE HTRA1 ; RETINAL-PIGMENT EPITHELIUM ; ENDOTHELIAL GROWTH-FACTOR ; MACULAR DEGENERATION ; GENE POLYMORPHISMS ; JAPANESE PATIENTS ; CHINESE PATIENTS ; A1 HTRA1 ; EXPRESSION
英文摘要

Multiple genetic studies have suggested that high-temperature requirement serine protease (HTRA1) is associated with polypoidal choroidal vasculopathy (PCV). To date, no functional studies have investigated the biological effect of HTRA1 on vascular endothelial cells, essential vascular components involved in polypoidal vascular abnormalities and arteriosclerosis-like changes. In vitro studies were performed to investigate the effect of HTRA1 on the regulation of fibronectin, laminin, vascular endothelial growth factor (VEGF), platelet derived growth factor receptor (PDGFR) and matrix metalloparoteinases 2 (MMP-2) and the role of HTRA1 in choroid-retina endothelial (RF/6A) and human umbilical vein endothelial (HUVEC) cells. Lentivirus-mediated overexpression of HTRA1 was used to explore effects of the protease on RF/6A and HUVEC cells in vitro. HTRA1 overexpression inhibited the proliferation, cell cycle, migration and tube formation of RF/6A and HUVEC cells, effects that might contribute to the early stage of PCV pathological lesions. Fibronectin mRNA and protein levels were significantly down-regulated following the upregulation of HTRA1, whereas the expressions of laminin, VEGF and MMP-2 were unaffected by alterations in HTRA1 expression. The decreased biological function of vascular endothelial cells and the degradation of extracellular matrix proteins, such as fibronectin, may be involved in a contributory role for HTRA1 in PCV pathogenesis.

语种英语
WOS记录号WOS:000309831500028
项目编号2011CB510200
资助机构National Basic Research Program of China (973 Program)
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59630
专题北京大学第二临床医学院_眼科
作者单位1.Minist Educ, Key Lab Vis Loss & Restorat, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China
3.China Japan Friendship Hosp, Dept Ophthalmol, Beijing, Peoples R China
4.Fudan Univ, Dept Ophthalmol, Eye & ENT Hosp, Shanghai 200433, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Jingjing,Huang, Lvzhen,Yu, Wenzhen,et al. Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs[J]. PLOS ONE,2012,7(10).
APA Jiang, Jingjing,Huang, Lvzhen,Yu, Wenzhen,Wu, Xi,Zhou, Peng,&Li, Xiaoxin.(2012).Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs.PLOS ONE,7(10).
MLA Jiang, Jingjing,et al."Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs".PLOS ONE 7.10(2012).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jiang, Jingjing]的文章
[Huang, Lvzhen]的文章
[Yu, Wenzhen]的文章
百度学术
百度学术中相似的文章
[Jiang, Jingjing]的文章
[Huang, Lvzhen]的文章
[Yu, Wenzhen]的文章
必应学术
必应学术中相似的文章
[Jiang, Jingjing]的文章
[Huang, Lvzhen]的文章
[Yu, Wenzhen]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。