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学科主题: 临床医学
题名:
Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs
作者: Jiang, Jingjing1,2,3; Huang, Lvzhen1,2; Yu, Wenzhen1,2; Wu, Xi1,2; Zhou, Peng1,2,4; Li, Xiaoxin1,2
刊名: PLOS ONE
发表日期: 2012-10-08
DOI: 10.1371/journal.pone.0046115
卷: 7, 期:10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: POLYPOIDAL CHOROIDAL VASCULOPATHY ; SERINE-PROTEASE HTRA1 ; RETINAL-PIGMENT EPITHELIUM ; ENDOTHELIAL GROWTH-FACTOR ; MACULAR DEGENERATION ; GENE POLYMORPHISMS ; JAPANESE PATIENTS ; CHINESE PATIENTS ; A1 HTRA1 ; EXPRESSION
英文摘要:

Multiple genetic studies have suggested that high-temperature requirement serine protease (HTRA1) is associated with polypoidal choroidal vasculopathy (PCV). To date, no functional studies have investigated the biological effect of HTRA1 on vascular endothelial cells, essential vascular components involved in polypoidal vascular abnormalities and arteriosclerosis-like changes. In vitro studies were performed to investigate the effect of HTRA1 on the regulation of fibronectin, laminin, vascular endothelial growth factor (VEGF), platelet derived growth factor receptor (PDGFR) and matrix metalloparoteinases 2 (MMP-2) and the role of HTRA1 in choroid-retina endothelial (RF/6A) and human umbilical vein endothelial (HUVEC) cells. Lentivirus-mediated overexpression of HTRA1 was used to explore effects of the protease on RF/6A and HUVEC cells in vitro. HTRA1 overexpression inhibited the proliferation, cell cycle, migration and tube formation of RF/6A and HUVEC cells, effects that might contribute to the early stage of PCV pathological lesions. Fibronectin mRNA and protein levels were significantly down-regulated following the upregulation of HTRA1, whereas the expressions of laminin, VEGF and MMP-2 were unaffected by alterations in HTRA1 expression. The decreased biological function of vascular endothelial cells and the degradation of extracellular matrix proteins, such as fibronectin, may be involved in a contributory role for HTRA1 in PCV pathogenesis.

语种: 英语
所属项目编号: 2011CB510200
项目资助者: National Basic Research Program of China (973 Program)
WOS记录号: WOS:000309831500028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59630
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Vis Loss & Restorat, Beijing, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China
3.China Japan Friendship Hosp, Dept Ophthalmol, Beijing, Peoples R China
4.Fudan Univ, Dept Ophthalmol, Eye & ENT Hosp, Shanghai 200433, Peoples R China

Recommended Citation:
Jiang, Jingjing,Huang, Lvzhen,Yu, Wenzhen,et al. Overexpression of HTRA1 Leads to Down-Regulation of Fibronectin and Functional Changes in RF/6A Cells and HUVECs[J]. PLOS ONE,2012,7(10).
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