北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔病理科  > 期刊论文
学科主题: 口腔医学
题名:
Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats
作者: Li, Binbin1; Liu, Hao2; Jia, Shengnan1
关键词: puerarin ; bone mass ; bone formation ; adiposity ; osteoporosis
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2014-12-01
卷: 37, 期:12, 页:1919-1925
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: ESTROGEN-RECEPTOR-BETA ; IN-VITRO ; EXPRESSION ; DIFFERENTIATION ; CELLS ; RADIX ; PHYTOESTROGENS ; ADIPOGENESIS ; RECRUITMENT ; PATHWAY
英文摘要:

We investigated the effect of puerarin on bone mass and marrow adiposity in ovariectomy (OVX)-induced osteoporosis. The rats were divided into four groups: control; OVX; OVX+estradiol (OVX-E); and OVX+puerarin treatment (OVX-GE). In vivo, bone mineral density (BMD) and histomorphometry were measured under microCT. The mechanical properties of tibia were obtained in 3-point bending test. Plasma osteocalcin and adiponectin were determined using enzyme-linked immunosorbent assay (ELISA). Alkaline phosphatase (ALP) were measured using biochemical methods. In vitro, 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) and Oil Red 0 staining were used to compare osteoblast proliferation and adipocyte differentiation, respectively. Osteocalcin and adiponectin in culture supernatants were determined using ELISA. The results showed that puerarin significantly enhanced bone volume density and trabecular number compared with OVX and OVX-E groups (p<0.05, p<0.05, respectively). Puerarin increased energy to ultimate load, plasma osteocalcin and ALP (p<0.01). However, BMD in OVX-GE group was less than that in control (p<0.01) and OVX-E groups (p<0.05). The culture supernatants from OVX-GE group showed increased osteocalcin compared with those from OVX (p<0.01) and OVX-E groups (p<0.05). Puerarin lowered adiponectin in culture supernatant compared with supernatant from OVX group and inhibited the increase in adipocytes caused by OVX (p<0.01). However, the amount of lipids did not differ between OVX-GE and OVX groups. These findings suggest that puerarin likely enhances bone formation by stimulating the proliferation and differentiation of osteoblasts while slightly inhibiting the adipotic differentiation.

语种: 英语
所属项目编号: 30901671
项目资助者: National Natural Science Foundations of China, Beijing, China
WOS记录号: WOS:000345495600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59662
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Li, Binbin,Liu, Hao,Jia, Shengnan. Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats[J]. BIOLOGICAL &amp; PHARMACEUTICAL BULLETIN,2014,37(12):1919-1925.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Binbin]'s Articles
[Liu, Hao]'s Articles
[Jia, Shengnan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Binbin]‘s Articles
[Liu, Hao]‘s Articles
[Jia, Shengnan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace