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Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats
Li, Binbin1; Liu, Hao2; Jia, Shengnan1
关键词puerarin bone mass bone formation adiposity osteoporosis
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2014-12-01
37期:12页:1919-1925
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]ESTROGEN-RECEPTOR-BETA ; IN-VITRO ; EXPRESSION ; DIFFERENTIATION ; CELLS ; RADIX ; PHYTOESTROGENS ; ADIPOGENESIS ; RECRUITMENT ; PATHWAY
英文摘要

We investigated the effect of puerarin on bone mass and marrow adiposity in ovariectomy (OVX)-induced osteoporosis. The rats were divided into four groups: control; OVX; OVX+estradiol (OVX-E); and OVX+puerarin treatment (OVX-GE). In vivo, bone mineral density (BMD) and histomorphometry were measured under microCT. The mechanical properties of tibia were obtained in 3-point bending test. Plasma osteocalcin and adiponectin were determined using enzyme-linked immunosorbent assay (ELISA). Alkaline phosphatase (ALP) were measured using biochemical methods. In vitro, 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) and Oil Red 0 staining were used to compare osteoblast proliferation and adipocyte differentiation, respectively. Osteocalcin and adiponectin in culture supernatants were determined using ELISA. The results showed that puerarin significantly enhanced bone volume density and trabecular number compared with OVX and OVX-E groups (p<0.05, p<0.05, respectively). Puerarin increased energy to ultimate load, plasma osteocalcin and ALP (p<0.01). However, BMD in OVX-GE group was less than that in control (p<0.01) and OVX-E groups (p<0.05). The culture supernatants from OVX-GE group showed increased osteocalcin compared with those from OVX (p<0.01) and OVX-E groups (p<0.05). Puerarin lowered adiponectin in culture supernatant compared with supernatant from OVX group and inhibited the increase in adipocytes caused by OVX (p<0.01). However, the amount of lipids did not differ between OVX-GE and OVX groups. These findings suggest that puerarin likely enhances bone formation by stimulating the proliferation and differentiation of osteoblasts while slightly inhibiting the adipotic differentiation.

语种英语
WOS记录号WOS:000345495600010
项目编号30901671
资助机构National Natural Science Foundations of China, Beijing, China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59662
专题北京大学口腔医学院_口腔病理科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
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GB/T 7714
Li, Binbin,Liu, Hao,Jia, Shengnan. Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats[J]. BIOLOGICAL &amp; PHARMACEUTICAL BULLETIN,2014,37(12):1919-1925.
APA Li, Binbin,Liu, Hao,&Jia, Shengnan.(2014).Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats.BIOLOGICAL & PHARMACEUTICAL BULLETIN,37(12),1919-1925.
MLA Li, Binbin,et al."Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats".BIOLOGICAL & PHARMACEUTICAL BULLETIN 37.12(2014):1919-1925.
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