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学科主题临床医学
Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis
Xin, G; Zhao, MH; Wang, HY
刊名CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
2004-05-01
DOI10.1128/CDLI.11.3.559-562.2004
11期:3页:559-562
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]SYSTEMIC VASCULITIS ; WEGENERS-GRANULOMATOSIS ; AUTOANTIBODIES ANCA ; INCREASING PROTEIN ; RENAL VASCULITIS ; EPIDEMIOLOGY ; DIAGNOSIS ; DISEASE
英文摘要

The detection rate of antineutrophil cytoplasmic antibodies (ANCA) in Chinese patients with clinically suspected small vessel vasculitis was investigated, and their antigen specificity and demographic features were analyzed. A number of sera (n = 5,604) sent to our referral laboratory for ANCA screening were tested by indirect immunofluorescence (IIF), enzyme-linked immunosorbent assays (ELISAs) for myeloperoxidase (MPO)- and proteinase 3 (PR3)-ANCA. Then the IIF-ANCA-positive sera that were negative for MPO- and PR3-ANCA were further tested by antigen-specific ELISA by using other five highly purified known ANCA antigens as solid-phase ligands. The known antigens included bactericidal/permeability-increasing protein (BPI), human leukocyte elastase (HLE), lactoferrin, cathepsin G, and azurocidins. Of the 5,604 sera, 267 (4.76%) sera were IIF-ANCA positive and 390 (7%) were antinuclear antibody (ANA) positive in the IIF assay. Of the IIF-positive samples, 213 were anti-MPO positive, 32 were anti-PR3 positive, and five cases were positive for both. Of the 48 sera positive for IIF-ANCA but negative for MPO- and PR3-ANCA, 13 sera (27%) recognized other target antigens, 7 sera recognized BPI, 5 recognized HLE, I recognize cathepsin G, and I recognized azurocidin. None of the sera recognized lactoferrin, and one serum sample recognized both BPI and HLE. The majority of ANCA-positive patients presented in summer or winter. There was no difference in gender (male/female ratio, 1:1.12) in ANCA-positive patients with a mean age of 53.1 years. The male/female ratio was 1.17:1 for patients over 60 years of age; however, it was 1:4 for patients under 20 years of age. We conclude that ANCA-related diseases are not rare in China, and the major antigens are MPO and PR3. When the IIF technique is used to detect ANCA, ANA should be carefully distinguished.

语种英语
WOS记录号WOS:000221570300016
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59664
专题北京大学第一临床医学院_肾脏内科
作者单位Peking Univ, Hosp 1, Dept Nephrol, Beijing 100034, Peoples R China
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GB/T 7714
Xin, G,Zhao, MH,Wang, HY. Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis[J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,2004,11(3):559-562.
APA Xin, G,Zhao, MH,&Wang, HY.(2004).Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis.CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,11(3),559-562.
MLA Xin, G,et al."Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis".CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 11.3(2004):559-562.
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