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学科主题: 临床医学
题名:
Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis
作者: Xin, G; Zhao, MH; Wang, HY
刊名: CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
发表日期: 2004-05-01
DOI: 10.1128/CDLI.11.3.559-562.2004
卷: 11, 期:3, 页:559-562
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]: Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]: SYSTEMIC VASCULITIS ; WEGENERS-GRANULOMATOSIS ; AUTOANTIBODIES ANCA ; INCREASING PROTEIN ; RENAL VASCULITIS ; EPIDEMIOLOGY ; DIAGNOSIS ; DISEASE
英文摘要:

The detection rate of antineutrophil cytoplasmic antibodies (ANCA) in Chinese patients with clinically suspected small vessel vasculitis was investigated, and their antigen specificity and demographic features were analyzed. A number of sera (n = 5,604) sent to our referral laboratory for ANCA screening were tested by indirect immunofluorescence (IIF), enzyme-linked immunosorbent assays (ELISAs) for myeloperoxidase (MPO)- and proteinase 3 (PR3)-ANCA. Then the IIF-ANCA-positive sera that were negative for MPO- and PR3-ANCA were further tested by antigen-specific ELISA by using other five highly purified known ANCA antigens as solid-phase ligands. The known antigens included bactericidal/permeability-increasing protein (BPI), human leukocyte elastase (HLE), lactoferrin, cathepsin G, and azurocidins. Of the 5,604 sera, 267 (4.76%) sera were IIF-ANCA positive and 390 (7%) were antinuclear antibody (ANA) positive in the IIF assay. Of the IIF-positive samples, 213 were anti-MPO positive, 32 were anti-PR3 positive, and five cases were positive for both. Of the 48 sera positive for IIF-ANCA but negative for MPO- and PR3-ANCA, 13 sera (27%) recognized other target antigens, 7 sera recognized BPI, 5 recognized HLE, I recognize cathepsin G, and I recognized azurocidin. None of the sera recognized lactoferrin, and one serum sample recognized both BPI and HLE. The majority of ANCA-positive patients presented in summer or winter. There was no difference in gender (male/female ratio, 1:1.12) in ANCA-positive patients with a mean age of 53.1 years. The male/female ratio was 1.17:1 for patients over 60 years of age; however, it was 1:4 for patients under 20 years of age. We conclude that ANCA-related diseases are not rare in China, and the major antigens are MPO and PR3. When the IIF technique is used to detect ANCA, ANA should be carefully distinguished.

语种: 英语
WOS记录号: WOS:000221570300016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59664
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: Peking Univ, Hosp 1, Dept Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Xin, G,Zhao, MH,Wang, HY. Detection rate and antigenic specificities of antineutrophil cytoplasmic antibodies in Chinese patients with clinically suspected vasculitis[J]. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY,2004,11(3):559-562.
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