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学科主题: 临床医学
题名:
Transplanted Endothelial Progenitor Cells Ameliorate Carbon Tetrachloride-Induced Liver Cirrhosis in Rats
作者: Liu, Feng; Liu, Zhi-Da; Wu, Nan; Cong, Xu; Fei, Ran; Chen, Hong-Song; Wei, Lai
刊名: LIVER TRANSPLANTATION
发表日期: 2009-09-01
DOI: 10.1002/lt.21845
卷: 15, 期:9, 页:1092-1100
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Surgery ; Transplantation
研究领域[WOS]: Gastroenterology & Hepatology ; Surgery ; Transplantation
关键词[WOS]: HEMATOPOIETIC STEM-CELLS ; HEPATOCYTE GROWTH-FACTOR ; HEPATIC-FIBROSIS ; GENE-THERAPY ; REGENERATION ; CONTRIBUTE ; SURVIVAL ; SCIENCE ; FUSION ; INJURY
英文摘要:

Cirrhosis is the most common end stage of liver diseases, and there are no effective treatment methods. Here we evaluated the effect of endothelial progenitor cell (EPC) transplantation from rat bone marrow (BM) on the development of cirrhosis induced by carbon tetrachloride (CCl(4)). Ex vivo generated, characterized, and cultivated rat BM-derived EPCs were identified by their vasculogenic properties in vitro. EPCs from male rats were transplanted into female rats via the intraportal vein 12 weeks after they had been challenged with CCl(4), and the rats were killed 16 weeks later. The control rats received only a saline infusion. The fibrosis index and donor cell engraftment were assessed after EPC transplantation. After transplantation via the portal vein, PKH26 labeling, polymerase chain reaction, and in situ hybridization analysis revealed that the donor EPCs had adhered to the vasolateral surfaces of blood vessels and established in the liver. EPCs reduced the expression of a-smooth muscle actin, collagen III, and transforming growth factor beta (P < 0.05) as well as levels of aspartate aminotransferase, alanine aminotransferase, and total bilirubin in the serum (P < 0.05), but at the same time they increased the levels of albumin and Ki67. CCl(4) treatment increased the international prothrombin ratio (P < 0.05) and reduced albumin levels, whereas EPCs restored these parameters to normal levels. These results suggest that EPC transplantation could play a role in regulating hepatocyte regeneration and ameliorating established liver cirrhosis. Liver Transpl 15:1092-1100, 2009. (C) 2009 AASLD.

语种: 英语
所属项目编号: 30700350 ; 2005CB522902 ; 2007CB512900
项目资助者: National Science Foundation Fund of China ; Major State Basic Research Development Program of China (973 Program)
WOS记录号: WOS:000269596700014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59680
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Liu, Feng,Liu, Zhi-Da,Wu, Nan,et al. Transplanted Endothelial Progenitor Cells Ameliorate Carbon Tetrachloride-Induced Liver Cirrhosis in Rats[J]. LIVER TRANSPLANTATION,2009,15(9):1092-1100.
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