北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 胃肠肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts
作者: Wang, Tingting; Gao, Jing; Yu, Jingwei; Shen, Lin
关键词: Wogonin (WOG) ; paclitaxel (PTX) ; gastric cancer ; synergistic effect
刊名: CHINESE JOURNAL OF CANCER RESEARCH
发表日期: 2013-10-01
DOI: 10.3978/j.issn.1000-9604.2013.08.14
卷: 25, 期:5, 页:505-513
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: ETOPOSIDE-INDUCED APOPTOSIS ; IN-VIVO ; FLAVONOID WOGONIN ; BLADDER-CANCER ; PLANT FLAVONE ; GROWTH ; CHEMOTHERAPY ; LYMPHOCYTES ; INDUCTION ; CISPLATIN
英文摘要:

Objective: To investigate the synergistic inhibitory effects of wogonin (WOG) and chemotherapeutic drugs on growth of gastric cancer cells and tumor xenografts.

Methods: The IC50 values of WOG, cisplatin (CDDP) and paclitaxel (PTX) in four gastric cancer cell lines were determined by MTS assay. Hoechst staining and the median effect method of Chou-Talalay were used to assess the apoptosis of cells and the interaction of two drugs, respectively. BGC-823-derived xenografts in nude mice were established to investigate the effects of WOG combined with chemotherapeutic drugs in vivo.

Results: WOG, CDDP and PTX inhibited the growth of BGC-823, MGC-803, MKN-45 and HGC-27 gastric cancer cells in a dose-dependent manner. WOG combined with CDDP or PTX synergistically inhibited the growth of all gastric cancer cell lines in vitro. In BGC-823, MGC-803, HGC-27 and MKN-45 cell lines, synergisms between WOG and PTX were shown when the fraction affected (Fa) values were <0.45, <0.90, <0.85 and <0.60. While WOG and CDDP had a synergistic inhibitory effect when the Fa values were >0, >0, >0.65 and >0.10. From the results of in vivo experiments using tumor xenografts, WOG and low-dose PTX showed better efficacy than either drug alone. The inhibitory percentages of tumor weight were 61.58%, 20.29%, and 22.28% for the combination, WOG-alone, and low-dose PTX-alone groups, respectively. Notably, WOG combined with CDDP displayed very high toxicity.

Conclusions: A synergistic inhibitory effect on growth was observed when WOG was combined with low-dose PTX in gastric cancer cells and tumor xenografts. These findings provide evidence for the design of a clinical trial to test the combination of WOG with low-dose PTX in human gastric cancer.

语种: 英语
WOS记录号: WOS:000326852400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59684
Appears in Collections:北京大学临床肿瘤学院_胃肠肿瘤中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China

Recommended Citation:
Wang, Tingting,Gao, Jing,Yu, Jingwei,et al. Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25(5):505-513.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Tingting]'s Articles
[Gao, Jing]'s Articles
[Yu, Jingwei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Tingting]‘s Articles
[Gao, Jing]‘s Articles
[Yu, Jingwei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace