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学科主题临床医学
Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts
Wang, Tingting; Gao, Jing; Yu, Jingwei; Shen, Lin
关键词Wogonin (WOG) paclitaxel (PTX) gastric cancer synergistic effect
刊名CHINESE JOURNAL OF CANCER RESEARCH
2013-10-01
DOI10.3978/j.issn.1000-9604.2013.08.14
25期:5页:505-513
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]ETOPOSIDE-INDUCED APOPTOSIS ; IN-VIVO ; FLAVONOID WOGONIN ; BLADDER-CANCER ; PLANT FLAVONE ; GROWTH ; CHEMOTHERAPY ; LYMPHOCYTES ; INDUCTION ; CISPLATIN
英文摘要

Objective: To investigate the synergistic inhibitory effects of wogonin (WOG) and chemotherapeutic drugs on growth of gastric cancer cells and tumor xenografts.

Methods: The IC50 values of WOG, cisplatin (CDDP) and paclitaxel (PTX) in four gastric cancer cell lines were determined by MTS assay. Hoechst staining and the median effect method of Chou-Talalay were used to assess the apoptosis of cells and the interaction of two drugs, respectively. BGC-823-derived xenografts in nude mice were established to investigate the effects of WOG combined with chemotherapeutic drugs in vivo.

Results: WOG, CDDP and PTX inhibited the growth of BGC-823, MGC-803, MKN-45 and HGC-27 gastric cancer cells in a dose-dependent manner. WOG combined with CDDP or PTX synergistically inhibited the growth of all gastric cancer cell lines in vitro. In BGC-823, MGC-803, HGC-27 and MKN-45 cell lines, synergisms between WOG and PTX were shown when the fraction affected (Fa) values were <0.45, <0.90, <0.85 and <0.60. While WOG and CDDP had a synergistic inhibitory effect when the Fa values were >0, >0, >0.65 and >0.10. From the results of in vivo experiments using tumor xenografts, WOG and low-dose PTX showed better efficacy than either drug alone. The inhibitory percentages of tumor weight were 61.58%, 20.29%, and 22.28% for the combination, WOG-alone, and low-dose PTX-alone groups, respectively. Notably, WOG combined with CDDP displayed very high toxicity.

Conclusions: A synergistic inhibitory effect on growth was observed when WOG was combined with low-dose PTX in gastric cancer cells and tumor xenografts. These findings provide evidence for the design of a clinical trial to test the combination of WOG with low-dose PTX in human gastric cancer.

语种英语
WOS记录号WOS:000326852400006
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59684
专题北京大学临床肿瘤学院_胃肠肿瘤中心
北京大学临床肿瘤学院_消化肿瘤内科
作者单位Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Wang, Tingting,Gao, Jing,Yu, Jingwei,et al. Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts[J]. CHINESE JOURNAL OF CANCER RESEARCH,2013,25(5):505-513.
APA Wang, Tingting,Gao, Jing,Yu, Jingwei,&Shen, Lin.(2013).Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts.CHINESE JOURNAL OF CANCER RESEARCH,25(5),505-513.
MLA Wang, Tingting,et al."Synergistic inhibitory effect of wogonin and low-dose paclitaxel on gastric cancer cells and tumor xenografts".CHINESE JOURNAL OF CANCER RESEARCH 25.5(2013):505-513.
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