学科主题临床医学
Rapid detection of hepatitis B virus surface antigen by an agglutination assay mediated by a bispecific diabody against both human erythrocytes and hepatitis B virus surface antigen
Chen, Yu-Ping; Qiao, Yuan-Yuan; Zhao, Xiao-Hang; Chen, Hong-Song; Wang, Yan; Wang, Zhuozhi
刊名CLINICAL AND VACCINE IMMUNOLOGY
2007-06-01
DOI10.1128/CVI.00310-06
14期:6页:720-725
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]SINGLE-CHAIN FV ; ESCHERICHIA-COLI ; ANTIBODY FRAGMENTS ; VARIABLE DOMAINS ; TUMOR-CELLS ; IN-VITRO ; BIVALENT ; MINIANTIBODIES ; CONSTRUCT ; AVIDITY
英文摘要

Bispecific antibodies have immense potential for use in clinical applications. In the present study, a bispecific diabody against human red blood cells (RBCs) and hepatitis B virus surface antigen (HBsAg) was used to detect HBsAg in blood specimens. The bispecific diabody was constructed by crossing over the variable region of the heavy chains and the light chains of anti-RBC and anti-HBsAg antibodies with a short linker, SRGGGS. In enzyme-linked immunosorbent assays, this bispecific diabody showed specific binding to both RBCs and HBsAg. When this bispecific diabody was mixed with human blood specimens in the presence of RBsAg, the dual binding sites of the diabody caused agglutination of human RBCs. This diabody-mediated agglutination assay was then used to test 712 clinical blood specimens and showed 97.7% sensitivity and 100% specificity when the results were compared with those of the conventional immunoassay, which was used as a reference. This autologous RBC agglutination assay provides a simple approach for rapid screening for HBsAg in blood specimens.

语种英语
WOS记录号WOS:000247769500011
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59689
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Navy Gen Hosp, Cent Lab, Beijing 100037, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Dermatol, Xian 710032, Peoples R China
3.Peking Univ, Peking Univ Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Inst Canc Res, Beijing, Peoples R China
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GB/T 7714
Chen, Yu-Ping,Qiao, Yuan-Yuan,Zhao, Xiao-Hang,et al. Rapid detection of hepatitis B virus surface antigen by an agglutination assay mediated by a bispecific diabody against both human erythrocytes and hepatitis B virus surface antigen[J]. CLINICAL AND VACCINE IMMUNOLOGY,2007,14(6):720-725.
APA Chen, Yu-Ping,Qiao, Yuan-Yuan,Zhao, Xiao-Hang,Chen, Hong-Song,Wang, Yan,&Wang, Zhuozhi.(2007).Rapid detection of hepatitis B virus surface antigen by an agglutination assay mediated by a bispecific diabody against both human erythrocytes and hepatitis B virus surface antigen.CLINICAL AND VACCINE IMMUNOLOGY,14(6),720-725.
MLA Chen, Yu-Ping,et al."Rapid detection of hepatitis B virus surface antigen by an agglutination assay mediated by a bispecific diabody against both human erythrocytes and hepatitis B virus surface antigen".CLINICAL AND VACCINE IMMUNOLOGY 14.6(2007):720-725.
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