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学科主题临床医学
Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer
Gu, Q; Wang, JD; Xia, HHX; Lin, MCM; He, H; Zou, B; Tu, SP; Yang, Y; Liu, XG; Lam, SK; Wong, WM; Chan, AOO; Yuen, MF; Kung, HF; Wong, BCY
刊名CARCINOGENESIS
2005-03-01
DOI10.1093/carcin/bgh345
26期:3页:541-546
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CYTOCHROME-C RELEASE ; CYCLOOXYGENASE-2 INHIBITOR ; MITOCHONDRIAL DYSFUNCTION ; CONFORMATIONAL-CHANGE ; DEATH RECEPTORS ; CELL-DEATH ; KAPPA-B ; PROTEIN ; TRANSLOCATION ; KINASE
英文摘要

Aspirin-induced apoptosis is one of the important mechanisms for its antitumour effect against gastric cancer. We aimed at investigating the involvement of bcl-2 family members in the apoptotic pathway in gastric cancer. Gastric cancer cell line AGS and MKN-45 were observed as to cell growth inhibition and induction of apoptosis in response to treatment with aspirin. Cell proliferation was measured by MTT assay. Apoptosis was determined by 4′-6-diamidino-2-phenylindole staining. Protein expression was determined by western blotting. We showed that aspirin activated caspase-8, caspase-9 and capase-3, cleaved and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome c release. In addition, suppression of caspase-8 with the specific inhibitor z-IETD-fmk, as well as the pan-caspase inhibitor z-VAD-fmk, prevented Bid cleavage and subsequent apoptosis. The caspase inhibitors failed to abolish the effects on Bax activation. In conclusion, our results identify a role of caspase-8/Bid and activation of Bax as a novel mechanism for aspirin-induced apoptosis in gastric cancer.

语种英语
WOS记录号WOS:000227242400004
引用统计
被引频次:68[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59734
专题北京大学第一临床医学院_消化科
作者单位1.Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100871, Peoples R China
3.Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China
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Gu, Q,Wang, JD,Xia, HHX,et al. Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer[J]. CARCINOGENESIS,2005,26(3):541-546.
APA Gu, Q.,Wang, JD.,Xia, HHX.,Lin, MCM.,He, H.,...&Wong, BCY.(2005).Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer.CARCINOGENESIS,26(3),541-546.
MLA Gu, Q,et al."Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer".CARCINOGENESIS 26.3(2005):541-546.
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