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学科主题: 临床医学
题名:
Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer
作者: Gu, Q; Wang, JD; Xia, HHX; Lin, MCM; He, H; Zou, B; Tu, SP; Yang, Y; Liu, XG; Lam, SK; Wong, WM; Chan, AOO; Yuen, MF; Kung, HF; Wong, BCY
刊名: CARCINOGENESIS
发表日期: 2005-03-01
DOI: 10.1093/carcin/bgh345
卷: 26, 期:3, 页:541-546
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CYTOCHROME-C RELEASE ; CYCLOOXYGENASE-2 INHIBITOR ; MITOCHONDRIAL DYSFUNCTION ; CONFORMATIONAL-CHANGE ; DEATH RECEPTORS ; CELL-DEATH ; KAPPA-B ; PROTEIN ; TRANSLOCATION ; KINASE
英文摘要:

Aspirin-induced apoptosis is one of the important mechanisms for its antitumour effect against gastric cancer. We aimed at investigating the involvement of bcl-2 family members in the apoptotic pathway in gastric cancer. Gastric cancer cell line AGS and MKN-45 were observed as to cell growth inhibition and induction of apoptosis in response to treatment with aspirin. Cell proliferation was measured by MTT assay. Apoptosis was determined by 4′-6-diamidino-2-phenylindole staining. Protein expression was determined by western blotting. We showed that aspirin activated caspase-8, caspase-9 and capase-3, cleaved and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome c release. In addition, suppression of caspase-8 with the specific inhibitor z-IETD-fmk, as well as the pan-caspase inhibitor z-VAD-fmk, prevented Bid cleavage and subsequent apoptosis. The caspase inhibitors failed to abolish the effects on Bax activation. In conclusion, our results identify a role of caspase-8/Bid and activation of Bax as a novel mechanism for aspirin-induced apoptosis in gastric cancer.

语种: 英语
WOS记录号: WOS:000227242400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59734
Appears in Collections:北京大学第一临床医学院_消化内科_期刊论文

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作者单位: 1.Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples R China
2.Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100871, Peoples R China
3.Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Gu, Q,Wang, JD,Xia, HHX,et al. Activation of the caspase-8/Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer[J]. CARCINOGENESIS,2005,26(3):541-546.
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