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学科主题: 临床医学
题名:
Proteome reference map and regulation network of neonatal rat cardiomyocyte
作者: Li, Zi-jian1,2,3; Liu, Ning4; Han, Qi-de1,2,3; Zhang, You-yi1,2,3
关键词: proteome ; neonatal rat cardiomyocyte ; regulation network ; 2-DE database
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2011-09-01
DOI: 10.1038/aps.2011.86
卷: 32, 期:9, 页:1116-1127
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: 2-DIMENSIONAL ELECTROPHORESIS DATABASE ; HEART PROTEINS ; IDENTIFICATION ; CONSTRUCTION ; HSC-2DPAGE ; MUSCLE ; DOG
英文摘要:

Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.

Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.

Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn.

Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.

语种: 英语
所属项目编号: 2011CB503903 ; 81070078 ; 81030001 ; 7102158
项目资助者: Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000294495700006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59749
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China
2.Jilin Univ, Hosp 2, Cent Lab, Changchun 130041, Peoples R China
3.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China

Recommended Citation:
Li, Zi-jian,Liu, Ning,Han, Qi-de,et al. Proteome reference map and regulation network of neonatal rat cardiomyocyte[J]. ACTA PHARMACOLOGICA SINICA,2011,32(9):1116-1127.
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