IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Proteome reference map and regulation network of neonatal rat cardiomyocyte
Li, Zi-jian1,2,3; Liu, Ning4; Han, Qi-de1,2,3; Zhang, You-yi1,2,3
关键词proteome neonatal rat cardiomyocyte regulation network 2-DE database
刊名ACTA PHARMACOLOGICA SINICA
2011-09-01
DOI10.1038/aps.2011.86
32期:9页:1116-1127
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
资助者Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]2-DIMENSIONAL ELECTROPHORESIS DATABASE ; HEART PROTEINS ; IDENTIFICATION ; CONSTRUCTION ; HSC-2DPAGE ; MUSCLE ; DOG
英文摘要

Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.

Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.

Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn.

Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.

语种英语
所属项目编号2011CB503903 ; 81070078 ; 81030001 ; 7102158
资助者Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; Major State Basic Research Development Program of China (973 Program) ; National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation
WOS记录号WOS:000294495700006
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59749
Collection北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China
2.Jilin Univ, Hosp 2, Cent Lab, Changchun 130041, Peoples R China
3.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Zi-jian,Liu, Ning,Han, Qi-de,et al. Proteome reference map and regulation network of neonatal rat cardiomyocyte[J]. ACTA PHARMACOLOGICA SINICA,2011,32(9):1116-1127.
APA Li, Zi-jian,Liu, Ning,Han, Qi-de,&Zhang, You-yi.(2011).Proteome reference map and regulation network of neonatal rat cardiomyocyte.ACTA PHARMACOLOGICA SINICA,32(9),1116-1127.
MLA Li, Zi-jian,et al."Proteome reference map and regulation network of neonatal rat cardiomyocyte".ACTA PHARMACOLOGICA SINICA 32.9(2011):1116-1127.
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