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学科主题: 临床医学
题名:
JNK-AP-1 pathway involved in interleukin-1 beta-induced calcitonin gene-related peptide secretion in human type II alveolar epithelial cells
作者: Li, Wenjing; Zheng, Shuai; Tang, Chaoshu; Zhu, Yi; Wang, Xian
关键词: CGRP ; IL-1AEII cell ; JNK ; AP-1
刊名: PEPTIDES
发表日期: 2007-06-01
DOI: 10.1016/j.peptides.2007.03.021
卷: 28, 期:6, 页:1252-1259
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: NERVE GROWTH-FACTOR ; RESPONSE ELEMENT ; SENSORY NEURONS ; KAPPA-B ; EXPRESSION ; KINASES ; RAT ; ACTIVATION ; NEUROPEPTIDES ; LYMPHOCYTES
英文摘要:

In a previous study, we found that calcitonin gene-related peptide (CGRP) could be induced by proinflammatory factor IL-1 in A549 human type II alveolar (AEII) epithelial cells. We investigated the mechanism of IL-1 beta-induced CGRP secretion and found that the PKC-p38 MAPK-NF-kappa B pathway was involved. in the present study, we found that IL-1 beta stimulation induced c-Jun N-terminal kinase (JNK) activity within 15 min in A549 cells. In investigating whether JNK was involved in IL-1 beta-induced CGRP secretion, the JNK II inhibitor SP600125 was used and it significantly attenuated IL-1 beta-induced CGRP secretion and c-Jun activity, which was elevated after IL-10 stimulation from mRNA to protein level. EMSA results showed the activation of activator protein 1 (AP-1) after 2-h IL-1 beta stimulation, and the JNK II inhibitor blocked c-Jun and AP-1 activity. Bioinformatic analysis showed five predicted AP-1 binding sites on the promoter of p-CGRP; deletion analysis identified an AP-1 consensus site at -643 bp relative to the initiation site, which mediates the beta-CGRP gene transcription in response to IL-10. These data suggest that besides the PKC-p38 MAPK-NF-kappa B pathway, the JNK-AP-1 pathway is involved in IL-1 beta-induced CGRP secretion in A549 human type II alveolar epithelial cells, and a 643-bp site upstream of the transcription start site on the promoter of beta-CGRP is the AP-1 response element. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000247701500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59761
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Sch Basic Med Sci,Reference Lab Educ Minist Mol C, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China

Recommended Citation:
Li, Wenjing,Zheng, Shuai,Tang, Chaoshu,et al. JNK-AP-1 pathway involved in interleukin-1 beta-induced calcitonin gene-related peptide secretion in human type II alveolar epithelial cells[J]. PEPTIDES,2007,28(6):1252-1259.
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