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学科主题: 临床医学
题名:
Application of an improved biuret method to the determination of total protein in urine and cerebrospinal fluid without concentration step by use of Hitachi 7170 auto-analyzer
作者: Xu, GB; Jiao, LL; Zhu, LH; Xia, T
关键词: protein ; CSF ; urine ; biuret
刊名: JOURNAL OF CLINICAL LABORATORY ANALYSIS
发表日期: 2001
卷: 15, 期:4, 页:161-164
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
英文摘要:

A biuret automated colorimetric assay for total protein in urine and cerebrospinal fluids was established. The procedures were as follows. Acidify all urine sample before analysis. Add precipitant Na2WO4 to urine samples. After 10 min, centrifuge, decant the supernatant fluid. drain the inverted tubes on absorbent tissue, dissolve the precipitation with 0.1 mol/L NaOH, and finally adapt the reconstituted urine to the Hitachi 7170 analyzer. A cell-free cerebrospinal fluid sample produced by centrifugation can be inserted in an auto-analyzer for protein measurement directly. The program: mix 35 mul sample (CSF or reconstituted urine) and standard with 0.2 mol/L NaOH; incurable at 37 degreesC for 5 min, and real A1. Add concentrated biuret reagent, and 10 min later measure absorbance A2 at 546 nm vs. reagent blank. Secondary wavelength was 700 nm. The test results were calculated against a one-point standard. This biuret colorimetric method was relatively simple, fast, and accurate for the determination of protein in urine and cerebrospinal fluid, with a wide linearity extending from 0.125 g/L up to 6 g/L, had a good correlation with Benzethonium chloride turbidimetry technique, and was a practical routine method. (C) 2001 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000169717600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59776
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Beijing Univ, Div Med, Hosp 1, Dept Clin Chem, Beijing 100034, Peoples R China

Recommended Citation:
Xu, GB,Jiao, LL,Zhu, LH,et al. Application of an improved biuret method to the determination of total protein in urine and cerebrospinal fluid without concentration step by use of Hitachi 7170 auto-analyzer[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2001,15(4):161-164.
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