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Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells
Zeng, Yan1,2; Liu, Hongtao1; Kang, Kang1; Wang, Zhiwei3; Hui, Gang4; Zhang, Xiaoying1; Zhong, Jiasheng1; Peng, Wenda2; Ramchandran, Ramaswamy5; Raj, J. Usha5; Gou, Deming1,5
刊名SCIENTIFIC REPORTS
2015-07-13
DOI10.1038/srep12098
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]BONE MORPHOGENETIC PROTEIN ; ENDOTHELIAL-CELLS ; CROSS-TALK ; HYPERTENSION ; RECEPTOR ; FIBROBLASTS ; DEFICIENCY ; MECHANISMS ; MUTATIONS ; MICRORNAS
英文摘要

There is growing evidence that microRNAs play important roles in cellular responses to hypoxia and in pulmonary hypertensive vascular remodeling, but the exact molecular mechanisms involved are not fully elucidated. In this study, we identified miR-322 as one of the microRNAs induced in lungs of chronically hypoxic mice and rats. The expression of miR-322 was also upregulated in primary cultured rat pulmonary arterial smooth muscle cells (PASMC) in response to hypoxia. We demonstrated that HIF-1 alpha, but not HIF-2 alpha, transcriptionally upregulates the expression of miR-322 in hypoxia. Furthermore, miR-322 facilitated the accumulation of HIF-1 alpha in the nucleus and promoted hypoxia-induced cell proliferation and migration. Direct targeting BMPR1a and smad5 by miR-322 was demonstrated in PASMCs suggesting that downregulation of BMP-Smad signaling pathway may be mediating the hypoxia-induced PASMC proliferation and migration. Our study implicates miR-322 in the hypoxic proliferative response of PASMCs suggesting that it may be playing a role in pulmonary vascular remodeling associated with pulmonary hypertension.

语种英语
WOS记录号WOS:000357849200001
项目编号81170047 ; 81370151 ; 2012CB124701 ; YFZZ20111009
资助机构National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Shenzhen overseas high-level talents innovation program
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59794
专题北京大学深圳医院
作者单位1.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresourse & Ecoenvironm, Shenzhen Key Lab Microbial Genet Engn, Shenzhen 518060, Guangdong, Peoples R China
2.Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Guangdong, Peoples R China
3.Shenzhen Sun Yat Sen Cardiovasc Hosp, Dept Cardiovasc Surg, Shenzhen 518000, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Chest Surg, Shenzhen 518000, Guangdong, Peoples R China
5.Univ Illinois, Dept Pediat, Chicago, IL 60612 USA
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GB/T 7714
Zeng, Yan,Liu, Hongtao,Kang, Kang,et al. Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells[J]. SCIENTIFIC REPORTS,2015,5.
APA Zeng, Yan.,Liu, Hongtao.,Kang, Kang.,Wang, Zhiwei.,Hui, Gang.,...&Gou, Deming.(2015).Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.SCIENTIFIC REPORTS,5.
MLA Zeng, Yan,et al."Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells".SCIENTIFIC REPORTS 5(2015).
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