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High-performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study
Yan, Haixia1,2; Wang, Li1; Li, Xingnuo2; Yu, Chun1; Zhang, Ke1; Jiang, Yong1; Wu, Lijun2; Lu, Wei1; Tu, Pengfei1
关键词carnosic acid HPLC pharmacokinetics bioavailability plasma
刊名BIOMEDICAL CHROMATOGRAPHY
2009-07-01
DOI10.1002/bmc.1184
23期:7页:776-781
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MASS SPECTROMETRIC ANALYSIS ; ROSMARINUS-OFFICINALIS L. ; DIODE-ARRAY DETECTION ; PHENOLIC DITERPENES ; CAPILLARY-ELECTROPHORESIS ; ROSEMARY EXTRACTS ; ANTIOXIDANTS ; SEPARATION
英文摘要

A sensitive and reproducible high-performance liquid chromatography method with ultraviolet detection W) was developed for the determination of carnosic acid (CA) in rat plasma. After simple acidification and liquid-liquid extraction of plasma samples using gemfibrozil as an internal standard, the supernatant was evaporated to dryness under a gentle stream of nitrogen. The residue was reconstituted in 200 mu L before being injected into the chromatographic system. The analysis was performed on a C(18) column protected by an ODS guard column using acetonitrile-0.1% phosphoric acid (55:45, v/v) as mobile phase, and the wavelength of the UV detector was set at 210 nm. The calibration curve was linear over the range of 0.265-265.0 g/mL with a correlation coefficient of 0.9997. The recovery for plasma samples of 0.530, 13.25, 132.5 and 265.0 g/mL was 72.2, 87.9, 90.4 and 94.7%, respectively. The intra-day and inter-day relative standard deviations for the measurements of quality control samples were less than 3.1%. The stability of the plasma samples was also validated. This method was successfully used to study the pharmacokinetics and bioavailability of CA in rats. Copyright (C) 2009 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000267302000014
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59799
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学药学院_药剂学系
北京大学第三临床医学院_耳鼻喉科
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang 110016, Peoples R China
推荐引用方式
GB/T 7714
Yan, Haixia,Wang, Li,Li, Xingnuo,et al. High-performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2009,23(7):776-781.
APA Yan, Haixia.,Wang, Li.,Li, Xingnuo.,Yu, Chun.,Zhang, Ke.,...&Tu, Pengfei.(2009).High-performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,23(7),776-781.
MLA Yan, Haixia,et al."High-performance liquid chromatography method for determination of carnosic acid in rat plasma and its application to pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 23.7(2009):776-781.
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