北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability
作者: Li, Jun1,2,3; Yan, Biao1,4; Huo, Zhaoxia1,4; Liu, Ying1,4; Xu, Jiahong2; Sun, Yunfu5; Liu, Yi1,3; Liang, Dandan1,3; Peng, Luying1,3; Zhang, Youyi6,7; Zhou, Zhao-Nian8; Shi, Jingyi9,10; Cui, Jianmin9,10; Chen, Yi-Han1,2,3
刊名: JOURNAL OF PHYSIOLOGY-LONDON
发表日期: 2010-08-15
DOI: 10.1113/jphysiol.2010.190900
卷: 588, 期:16, 页:2987-2998
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences ; Physiology
研究领域[WOS]: Neurosciences & Neurology ; Physiology
关键词[WOS]: NITRIC-OXIDE ; ADRENERGIC-RECEPTORS ; ADENYLYL-CYCLASE ; PROTEIN-KINASE ; MITOCHONDRIA ; HYPOXIA ; PATHWAY ; GENE ; LOCALIZATION ; STIMULATION
英文摘要:

beta-Adrenoceptors (beta-ARs) play a critical role in the regulation of cardiovascular function. Intracellular oxygen homeostasis is crucial for the survival of cardiomyocytes. However, it is still unclear whether beta-AR activation can modulate intracellular oxygen. Here we used mitochondrial and cytosolic target Renilla luciferase to detect intracellular oxygen concentration. Pharmacological experiments revealed that beta(2)-AR activation specifically regulates intracellular oxygen in cardiomyocytes and COS7 cells. This effect was abrogated by inhibitory G protein (G(i)) inhibition, endothelial nitric oxide synthase (eNOS) blockade, and NO scavenging, implicating that the beta(2)-AR-G(i)-eNOS pathway is involved in this regulation. beta(2)-AR activation increased the AMP/ATP ratio, AMPK activity, ROS production and prolyl hydroxylase activity. These effects also contribute to the regulation of beta(2)-AR signalling, thus providing an additional layer of complexity to enforce the specificity of beta(1)-AR and beta(2)-AR signalling. Collectively, the study provides novel insight into the modulation of oxygen homeostasis, broadens the scope of beta(2)-AR function, and may have crucial implications for beta(2)-AR signalling regulation.

语种: 英语
所属项目编号: 30425016 ; 30330290 ; 30470961 ; 30528011 ; 2007CB512100 ; 2006CB504100 ; 2007AA02Z438 ; 07PJ14058
项目资助者: National Science Fund of China ; &prime ; 973&prime ; Program Fund of China ; &prime ; 863&prime ; Program Fund of China ; Ministry of Education of China ; Shanghai Pujiang Program Fund ; Program Fund for Shanghai Subject Chief Scientists
WOS记录号: WOS:000281001900004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59802
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Tongji Univ, Inst Med Genet, Shanghai 200120, Peoples R China
2.Washington Univ, Arrhythmia Ctr, St Louis, MO 63130 USA
3.Tongji Univ, Sch Med, Minist Educ, Key Lab Arrhythmias,East Hosp, Shanghai 200120, Peoples R China
4.Tongji Univ, Sch Med, Dept Cardiol, East Hosp, Shanghai 200120, Peoples R China
5.Tongji Univ, Dept Biochem & Mol Biol, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
6.Univ Calif San Diego, Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
7.Peking Univ, Hosp 3, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
8.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
9.Chinese Acad Sci, Shanghai Inst Biol Sci, Lab Hypox Cardiovasc Physiol, Shanghai 200031, Peoples R China
10.Washington Univ, Dept Biomed Engn & Cardiac Bioelect, St Louis, MO 63130 USA

Recommended Citation:
Li, Jun,Yan, Biao,Huo, Zhaoxia,et al. beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability[J]. JOURNAL OF PHYSIOLOGY-LONDON,2010,588(16):2987-2998.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Jun]'s Articles
[Yan, Biao]'s Articles
[Huo, Zhaoxia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Jun]‘s Articles
[Yan, Biao]‘s Articles
[Huo, Zhaoxia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace