IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability
Li, Jun1,2,3; Yan, Biao1,4; Huo, Zhaoxia1,4; Liu, Ying1,4; Xu, Jiahong2; Sun, Yunfu5; Liu, Yi1,3; Liang, Dandan1,3; Peng, Luying1,3; Zhang, Youyi6,7; Zhou, Zhao-Nian8; Shi, Jingyi9,10; Cui, Jianmin9,10; Chen, Yi-Han1,2,3
刊名JOURNAL OF PHYSIOLOGY-LONDON
2010-08-15
DOI10.1113/jphysiol.2010.190900
588期:16页:2987-2998
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences ; Physiology
研究领域[WOS]Neurosciences & Neurology ; Physiology
关键词[WOS]NITRIC-OXIDE ; ADRENERGIC-RECEPTORS ; ADENYLYL-CYCLASE ; PROTEIN-KINASE ; MITOCHONDRIA ; HYPOXIA ; PATHWAY ; GENE ; LOCALIZATION ; STIMULATION
英文摘要

beta-Adrenoceptors (beta-ARs) play a critical role in the regulation of cardiovascular function. Intracellular oxygen homeostasis is crucial for the survival of cardiomyocytes. However, it is still unclear whether beta-AR activation can modulate intracellular oxygen. Here we used mitochondrial and cytosolic target Renilla luciferase to detect intracellular oxygen concentration. Pharmacological experiments revealed that beta(2)-AR activation specifically regulates intracellular oxygen in cardiomyocytes and COS7 cells. This effect was abrogated by inhibitory G protein (G(i)) inhibition, endothelial nitric oxide synthase (eNOS) blockade, and NO scavenging, implicating that the beta(2)-AR-G(i)-eNOS pathway is involved in this regulation. beta(2)-AR activation increased the AMP/ATP ratio, AMPK activity, ROS production and prolyl hydroxylase activity. These effects also contribute to the regulation of beta(2)-AR signalling, thus providing an additional layer of complexity to enforce the specificity of beta(1)-AR and beta(2)-AR signalling. Collectively, the study provides novel insight into the modulation of oxygen homeostasis, broadens the scope of beta(2)-AR function, and may have crucial implications for beta(2)-AR signalling regulation.

语种英语
WOS记录号WOS:000281001900004
项目编号30425016 ; 30330290 ; 30470961 ; 30528011 ; 2007CB512100 ; 2006CB504100 ; 2007AA02Z438 ; 07PJ14058
资助机构National Science Fund of China ; &prime ; 973&prime ; Program Fund of China ; &prime ; 863&prime ; Program Fund of China ; Ministry of Education of China ; Shanghai Pujiang Program Fund ; Program Fund for Shanghai Subject Chief Scientists
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59802
专题北京大学第三临床医学院_心血管内科
作者单位1.Tongji Univ, Inst Med Genet, Shanghai 200120, Peoples R China
2.Washington Univ, Arrhythmia Ctr, St Louis, MO 63130 USA
3.Tongji Univ, Sch Med, Minist Educ, Key Lab Arrhythmias,East Hosp, Shanghai 200120, Peoples R China
4.Tongji Univ, Sch Med, Dept Cardiol, East Hosp, Shanghai 200120, Peoples R China
5.Tongji Univ, Dept Biochem & Mol Biol, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
6.Univ Calif San Diego, Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
7.Peking Univ, Hosp 3, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
8.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
9.Chinese Acad Sci, Shanghai Inst Biol Sci, Lab Hypox Cardiovasc Physiol, Shanghai 200031, Peoples R China
10.Washington Univ, Dept Biomed Engn & Cardiac Bioelect, St Louis, MO 63130 USA
推荐引用方式
GB/T 7714
Li, Jun,Yan, Biao,Huo, Zhaoxia,et al. beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability[J]. JOURNAL OF PHYSIOLOGY-LONDON,2010,588(16):2987-2998.
APA Li, Jun.,Yan, Biao.,Huo, Zhaoxia.,Liu, Ying.,Xu, Jiahong.,...&Chen, Yi-Han.(2010).beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability.JOURNAL OF PHYSIOLOGY-LONDON,588(16),2987-2998.
MLA Li, Jun,et al."beta(2)- but not beta(1)-adrenoceptor activation modulates intracellular oxygen availability".JOURNAL OF PHYSIOLOGY-LONDON 588.16(2010):2987-2998.
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