|Expression of the MAGE-1 gene in human hepatocellular carcinomas|
|Chen, HS; Cai, SL; Wang, Y; Zhao, HT; Peng, J; Pang, XW; Zhu, JY; Cong, Y; Rui, J; Leng, XS; Du, RY; Wang, Y; Vaughan, H; Cebon, J; Burgess, AW; Chen, WF|
|关键词||hepatocellular carcinoma melanoma antigen|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||T-LYMPHOCYTES ; NONAPEPTIDE ; MELANOMA ; HLA-A2 ; FAMILY|
Objective To further investigate the expression of MAGE-1 gene in hepatocellular carcinoma (HCC).
Methods The tumors and adjacent liver tissue from 45 HCC patients and liver tissue from 28 non-HCC patients (16 with liver cirrhosis and 12 with normal liver) were characterized by RT-PCR. A 421 bp PCR product from a cDNA fragment spanning exons 1, 2 and 3 was sequenced. The HLA type was assayed by standard ELISA in 43 HCC patients.
Results Thirty-two of 45 tumor tissues from HCC patients expressed MAGE-1 mRNA (71.1%). In contrast, MAGE-1 mRNA was not detected in adjacent tissues. Three were found to have point mutations at 3 identical sites resulting in the substitution of two amino acid residues. The most frequent HLA types in 43 HCC patients were: HLA-A2, 53.5%; A11, 25.6%; A24, 20.9%; A33, 20.9%; HLA-B13, 28.3% and B35, 23.2%. Expression of HLA-A33 (20.9%) was higher in HCC patients than that predicted in the normal Chinese population (8.8%). There was no discernable correlation between MAGE-1 expression and alpha -FP level, tumor size and hepatitis B or C virus infection. The identification of peptides which are restricted by haploptypes other than A1 should increase the opportunity for peptide based immunotherapy.
Conclusions This study shows that MAGE-1 mRNA is highly expressed in HCC tumor tissue in Chinese patients. Previously unreported point mutations in the MAGE-1 gene are described and may also provide additional opportunities for immunotherapy.
|作者单位||1.Peking Univ, Dept Immunol, Beijing 100083, Peoples R China|
2.Peking Univ, Sch Basic Med Sci, Liver Canc Res Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Dept Surg, Beijing 100044, Peoples R China
5.Ludwig Inst Canc Res, Melbourne Branch, Heidelberg, Vic 3085, Australia
6.Peking Univ, Dept Immunol, Beijing 100083, Peoples R China
7.Peking Univ, Sch Basic Med Sci, Liver Canc Res Ctr, Beijing 100083, Peoples R China
8.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
9.Peking Univ, Peoples Hosp, Dept Surg, Beijing 100044, Peoples R China
10.Ludwig Inst Canc Res, Melbourne Branch, Heidelberg, Vic 3085, Australia
|Chen, HS,Cai, SL,Wang, Y,et al. Expression of the MAGE-1 gene in human hepatocellular carcinomas[J]. CHINESE MEDICAL JOURNAL,2000,113(12):1112-1118.|
|APA||Chen, HS.,Cai, SL.,Wang, Y.,Zhao, HT.,Peng, J.,...&Chen, WF.(2000).Expression of the MAGE-1 gene in human hepatocellular carcinomas.CHINESE MEDICAL JOURNAL,113(12),1112-1118.|
|MLA||Chen, HS,et al."Expression of the MAGE-1 gene in human hepatocellular carcinomas".CHINESE MEDICAL JOURNAL 113.12(2000):1112-1118.|
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