Institutional Repository of Peking University School of Basic Medical Sciences
|Transcriptome Analysis Reveals Distinct Patterns of Long Noncoding RNAs in Heart and Plasma of Mice with Heart Failure|
|Li, Danhua1,4; Chen, Geng2,4; Yang, Jichun1,4; Fan, Xiaofang5; Gong, Yongsheng5; Xu, Guoheng1,4; Cui, Qinghua1,3,4; Geng, Bin1,4|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||HUMAN-DISEASE ; BIOMARKERS ; NEURODEGENERATION ; EXPRESSION|
Objective: To assess the global changes in and characteristics of the transcriptome of long noncoding RNAs (LncRNAs) in heart tissue, whole blood and plasma during heart failure (HF) and association with expression of paired coding genes.
Methods: Here we used microarray assay to examine the transcriptome of LncRNAs deregulated in the heart, whole blood, and plasma during HF in mice. We confirmed the changes in LncRNAs by quantitative PCR.
Results: We revealed and confirmed a number of LncRNAs that were deregulated during HF, which suggests a potential role of LncRNAs in HF. Strikingly, the patterns of expression of LncRNA differed between plasma and other tissue during HF. LncRNA expression was associated with LncRNA length in all samples but not in plasma during HF, which suggests that the global association of LncRNA expression and LncRNA length in plasma could be biomarkers for HF. In total, 32 LncRNAs all expressed in the heart, whole blood and plasma showed changed expression with HF, so they may be biomarkers in HF. In addition, sense-overlapped LncRNAs tended to show consistent expression with their paired coding genes, whereas antisense-overlapped LncRNAs tended to show the opposite expression in plasma; so different types of LncRNAs may have different characteristics in HF. Interestingly, we revealed an inverse correlation between changes in expression of LncRNAs in plasma and in heart, so circulating levels of LncRNAs may not represent just passive leakage from the HF heart but also active regulation or release of circulatory cells or other cells during HF.
Conclusions: We reveal stable expression of LncRNAs in plasma during HF, which suggests a newly described component in plasma. The distinct expression patterns of circulatory LncRNAs during HF indicate that LncRNAs may actively respond to stress and thus serve as biomarkers of HF diagnosis and treatment.
|项目编号||2012CB517806 ; 2012CB5106 ; 2009CB941603 ; 81170235 ; 81070114|
|资助机构||Major State Basic Research Development Program of China ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University|
|作者单位||1.Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Beijing 100871, Peoples R China|
2.Peking Univ, Sch Basic Med Sci, Dept Biomed Informat, Beijing 100871, Peoples R China
3.Peking Univ, Inst Syst Biomed, Beijing 100871, Peoples R China
4.Peking Univ, MOE Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
5.Wenzhou Med Univ, Inst Hypoxia Med, Wenzhou, Zhejiang, Peoples R China
|Li, Danhua,Chen, Geng,Yang, Jichun,et al. Transcriptome Analysis Reveals Distinct Patterns of Long Noncoding RNAs in Heart and Plasma of Mice with Heart Failure[J]. PLOS ONE,2013,8(10).|
|APA||Li, Danhua.,Chen, Geng.,Yang, Jichun.,Fan, Xiaofang.,Gong, Yongsheng.,...&Geng, Bin.(2013).Transcriptome Analysis Reveals Distinct Patterns of Long Noncoding RNAs in Heart and Plasma of Mice with Heart Failure.PLOS ONE,8(10).|
|MLA||Li, Danhua,et al."Transcriptome Analysis Reveals Distinct Patterns of Long Noncoding RNAs in Heart and Plasma of Mice with Heart Failure".PLOS ONE 8.10(2013).|
|Transcriptome Analys（1223KB）||期刊论文||出版稿||开放获取||CC BY-NC-SA||浏览 下载|