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Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway
Liu, Zhongning1; Jiang, Ting1; Wang, Xinzhi1; Wang, Yixiang2
关键词fluocinolone acetonide inflammation mineralization dental pulp cells
刊名BRITISH JOURNAL OF PHARMACOLOGY
2013-11-01
DOI10.1111/bph.12404
170期:6页:1262-1271
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]RETINOIC ACID ; TNF-ALPHA ; IN-VITRO ; INFLAMMATION ; BONE ; LIPOPOLYSACCHARIDE ; EXPRESSION ; TISSUE ; STRATEGIES ; RECEPTOR
英文摘要

Background and PurposeFluocinolone acetonide (FA) is commonly used as a steroidal anti-inflammatory drug. We recently found that in dental pulp cells (DPCs) FA has osteo-/odonto-inductive as well as anti-inflammatory effects. However, the mechanism by which FA induces these effects in DPCs is poorly understood.

Experimental ApproachThe effect of FA on the mineralization of DPCs during inflammatory conditions and the underlying mechanism were investigated by real-time PCR, Western blot, EMSA, histochemical staining, immunostaining and pathway blockade assays.

Key ResultsFA significantly inhibited the inflammatory response in LPS-treated DPCs not only by down-regulating the expression of pro-inflammation-related genes, but also by up-regulating the expression of the anti-inflammatory gene PPAR- and mineralization-related genes. Moreover, histochemical staining and immunostaining showed that FA could partially restore the expressions of alkaline phosphatase, osteocalcin and dentin sialophosphoprotein (DSPP) and mineralization in LPS-stimulated DPCs. Real-time PCR and Western blot analysis revealed that FA up-regulated DSPP and runt-related transcription factor 2 expression by inhibiting the expression of phosphorylated-NF-B P65 and activating activator protein-1 (AP-1) (p-c-Jun and Fra-1). These results were further confirmed through EMSA, by detection of NF-B DNA-binding activity and pathway blockade assays using a NF-B pathway inhibitor, AP-1 pathway inhibitor and glucocorticoid receptor antagonist.

Conclusions and ImplicationsInflammation induced by LPS suppresses the mineralization process in DPCs. FA partially restored this osteo-/odonto-genesis process in LPS-treated DPCs and had an anti-inflammatory effect through inhibition of the NF-B pathway and activation of the AP-1 pathway. Hence, FA is a potential new treatment for inflammation-associated bone/teeth diseases.

语种英语
WOS记录号WOS:000326121300011
项目编号30973353 ; 81172556
资助机构National Natural Science Foundation of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59851
专题北京大学口腔医学院_口腔修复科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
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GB/T 7714
Liu, Zhongning,Jiang, Ting,Wang, Xinzhi,et al. Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway[J]. BRITISH JOURNAL OF PHARMACOLOGY,2013,170(6):1262-1271.
APA Liu, Zhongning,Jiang, Ting,Wang, Xinzhi,&Wang, Yixiang.(2013).Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway.BRITISH JOURNAL OF PHARMACOLOGY,170(6),1262-1271.
MLA Liu, Zhongning,et al."Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway".BRITISH JOURNAL OF PHARMACOLOGY 170.6(2013):1262-1271.
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