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学科主题: 口腔医学
题名:
Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway
作者: Liu, Zhongning1; Jiang, Ting1; Wang, Xinzhi1; Wang, Yixiang2
关键词: fluocinolone ; acetonide ; inflammation ; mineralization ; dental pulp cells
刊名: BRITISH JOURNAL OF PHARMACOLOGY
发表日期: 2013-11-01
DOI: 10.1111/bph.12404
卷: 170, 期:6, 页:1262-1271
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: RETINOIC ACID ; TNF-ALPHA ; IN-VITRO ; INFLAMMATION ; BONE ; LIPOPOLYSACCHARIDE ; EXPRESSION ; TISSUE ; STRATEGIES ; RECEPTOR
英文摘要:

Background and PurposeFluocinolone acetonide (FA) is commonly used as a steroidal anti-inflammatory drug. We recently found that in dental pulp cells (DPCs) FA has osteo-/odonto-inductive as well as anti-inflammatory effects. However, the mechanism by which FA induces these effects in DPCs is poorly understood.

Experimental ApproachThe effect of FA on the mineralization of DPCs during inflammatory conditions and the underlying mechanism were investigated by real-time PCR, Western blot, EMSA, histochemical staining, immunostaining and pathway blockade assays.

Key ResultsFA significantly inhibited the inflammatory response in LPS-treated DPCs not only by down-regulating the expression of pro-inflammation-related genes, but also by up-regulating the expression of the anti-inflammatory gene PPAR- and mineralization-related genes. Moreover, histochemical staining and immunostaining showed that FA could partially restore the expressions of alkaline phosphatase, osteocalcin and dentin sialophosphoprotein (DSPP) and mineralization in LPS-stimulated DPCs. Real-time PCR and Western blot analysis revealed that FA up-regulated DSPP and runt-related transcription factor 2 expression by inhibiting the expression of phosphorylated-NF-B P65 and activating activator protein-1 (AP-1) (p-c-Jun and Fra-1). These results were further confirmed through EMSA, by detection of NF-B DNA-binding activity and pathway blockade assays using a NF-B pathway inhibitor, AP-1 pathway inhibitor and glucocorticoid receptor antagonist.

Conclusions and ImplicationsInflammation induced by LPS suppresses the mineralization process in DPCs. FA partially restored this osteo-/odonto-genesis process in LPS-treated DPCs and had an anti-inflammatory effect through inhibition of the NF-B pathway and activation of the AP-1 pathway. Hence, FA is a potential new treatment for inflammation-associated bone/teeth diseases.

语种: 英语
所属项目编号: 30973353 ; 81172556
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000326121300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59851
Appears in Collections:北京大学口腔医学院_口腔修复科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Liu, Zhongning,Jiang, Ting,Wang, Xinzhi,et al. Fluocinolone acetonide partially restores the mineralization of LPS-stimulated dental pulp cells through inhibition of NF-kappa B pathway and activation of AP-1 pathway[J]. BRITISH JOURNAL OF PHARMACOLOGY,2013,170(6):1262-1271.
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