|Antiviral action of interferon-alpha against hepatitis C virus replicon and its modulation by interferon-gamma and interleukin-8|
|Jia, Yintang; Wei, Lai; Jiang, Dong; Wang, Jianghua; Cong, Xu; Fei, Ran|
|关键词||hepatitis C virus replicon interferon-alpha interferon-gamma interleukin-8|
|刊名||JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||NONSTRUCTURAL 5A PROTEIN ; CELL-CULTURE ; RNA REPLICATION ; PLUS RIBAVIRIN ; EXPRESSION ; INFECTION ; NS5A ; RESISTANCE ; MUTATIONS ; THERAPY|
Background and Aim: Interferon-a (IFN-alpha) based therapy is the main treatment used to control hepatitis C virus (HCV) infection. The aim of this study was to understand the mechanisms of IFN-alpha inhibition of HCV replication and the resistance of HCV to IFN-alpha therapy, and improve the efficiency of HCV treatment.
Methods: The inhibitory effects of IFN-a on a HCV replicon system were examined and the potential regulatory effects of interferon-gamma (IFN-gamma) and interleukin-8 (IL-8) on the antiviral actions of IFN-a were also investigated in this report.
Results: The results showed that IFN-alpha can effectively inhibit the replication of HCV replicon. Pretreatment of HCV replicon cells with IFN-gamma could significantly potentiate the inhibitory effects of IFN-alpha on the HCV replicon. Direct addition of IL-8 to the culture medium of HCV replicon cells could partially rescue the HCV replicon from the inhibition of IFN-alpha, which may be the result of IL-8 down-regulation of interferon-stimulated genes.
Conclusion: Our study demonstrated that IFN-gamma has synergistic antiviral effects with IFN-alpha; whereas IL-8 can attenuate the anti-HCV actions of IFN-alpha and is associated with HCV resistance to interferon-alpha therapy.
|作者单位||Peking Univ, People Hosp, Inst Hepatol, Beijing 100044, Peoples R China|
|Jia, Yintang,Wei, Lai,Jiang, Dong,et al. Antiviral action of interferon-alpha against hepatitis C virus replicon and its modulation by interferon-gamma and interleukin-8[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2007,22(8):1278-1285.|
|APA||Jia, Yintang,Wei, Lai,Jiang, Dong,Wang, Jianghua,Cong, Xu,&Fei, Ran.(2007).Antiviral action of interferon-alpha against hepatitis C virus replicon and its modulation by interferon-gamma and interleukin-8.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,22(8),1278-1285.|
|MLA||Jia, Yintang,et al."Antiviral action of interferon-alpha against hepatitis C virus replicon and its modulation by interferon-gamma and interleukin-8".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 22.8(2007):1278-1285.|