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学科主题基础医学
A novel tumor metastasis suppressor gene LASS2/TMSG1 interacts with vacuolar ATPase through its homeodomain
Yu, Wenjuan1,2; Wang, Leiming1; Wang, Yuewei3; Xu, Xiaoyan1; Zou, Pengcheng1; Gong, Miaozi1; Zheng, Jie1; You, Jiangfeng1; Wang, Hua1; Mei, Fang1; Pei, Fei1
关键词TUMOR METASTASIS SUPPRESSOR GENE LASS2/TMSG1 PROSTATE CANCER VACUOLAR ATPASE HOMEODOMAIN
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2013-03-01
DOI10.1002/jcb.24400
114期:3页:570-583
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]CERAMIDE ; CANCER ; INHIBITION ; CDX2
英文摘要

LASS2/TMSG1 was a novel tumor metastasis suppressor gene, which was first cloned by our laboratory from non-metastatic and metastatic cancer cell variants of human prostate carcinoma PC-3M using mRNA differential display in 1999. LASS2/TMSG1 could interact with the C subunit of vacuolar ATPase (V-ATPase, ATP6V0C) and regulate V-ATPase activity. In an attempt to provide molecular mechanism of the interaction between LASS2/TMSG1 and V-ATPase, we constructed four variant transfectants containing different functional domain of LASS2/TMSG1 and stably transfected the variants to human prostate cancer cell line PC-3M-1E8 cell with high metastatic potential. Results showed that there were no obvious differences of V-ATPase expression among different transfected cells and the control. However, V-ATPase activity and intracellular pH was significantly higher in the variant transfectants with Homeodomain of LASS2/TMSG1 than that in the control using the pH-dependent fluorescence probe BECEF/AM. Immunoprecipitation, immunofluorescence and immuno-electron microscope alone or in combination demonstrated the direct interaction of Homeodomain of LASS2/TMSG1 and ATP6V0C. Loss of Homeodomain markedly enhanced the proliferation ability but weakened the apoptotic effect of LASS2/TMSG1 in PC-3M-1E8 cells. These lines of results for the first time contribute to the conclusion that LASS2/TMSG1 could regulate V-ATPase activity and intracellular pH through the direct interaction of its Homeodomain and the C subunit of V-ATPase. Their interaction could play important roles in the apoptosis of tumor cells. J. Cell. Biochem. 114: 570583, 2013. (C) 2012 Wiley Periodicals, Inc.

语种英语
WOS记录号WOS:000313900600010
项目编号30971142
资助机构National Sciences Foundation of China
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59868
专题北京大学基础医学院_病理学系
北京大学基础医学院
北京大学医学图书馆_流通阅览部
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China
2.Qingdao Univ, Coll Med, Affiliated Hosp, Dept Pathol, Qingdao 266003, Peoples R China
3.Qingdao Univ, Affiliated Hosp, Coll Med, Dept Vasc Surg, Qingdao 266003, Peoples R China
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Yu, Wenjuan,Wang, Leiming,Wang, Yuewei,et al. A novel tumor metastasis suppressor gene LASS2/TMSG1 interacts with vacuolar ATPase through its homeodomain[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2013,114(3):570-583.
APA Yu, Wenjuan.,Wang, Leiming.,Wang, Yuewei.,Xu, Xiaoyan.,Zou, Pengcheng.,...&Pei, Fei.(2013).A novel tumor metastasis suppressor gene LASS2/TMSG1 interacts with vacuolar ATPase through its homeodomain.JOURNAL OF CELLULAR BIOCHEMISTRY,114(3),570-583.
MLA Yu, Wenjuan,et al."A novel tumor metastasis suppressor gene LASS2/TMSG1 interacts with vacuolar ATPase through its homeodomain".JOURNAL OF CELLULAR BIOCHEMISTRY 114.3(2013):570-583.
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