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学科主题: 临床医学
题名:
H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion
作者: Yi, Li1; Zhang, Haiou1; Lei, Hao2; Wei, Li2
关键词: cerebral ischemia ; H-1-MRS ; neuroprotection ; vascular endothelial growth factor
刊名: NEURAL REGENERATION RESEARCH
发表日期: 2008-10-01
卷: 3, 期:10, 页:1116-1120
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Neurosciences
研究领域[WOS]: Cell Biology ; Neurosciences & Neurology
关键词[WOS]: REDUCE INFARCTION VOLUME ; BRAIN-BARRIER DISRUPTION ; ARTERY OCCLUSION ; VEGF ; RAT ; DAMAGE ; MICE ; ISCHEMIA/REPERFUSION ; ANGIOGENESIS ; PERMEABILITY
英文摘要:

BACKGROUND: It has become generally accepted that measuring N-acetyl-L-aspartic acid through the use of H-1-magnetic resonance spectroscopy (H-1-MRS) could be used to evaluate neuronal injury.

OBJECTIVE: To study metabolic changes of N-acetyl-L-aspartic acid surrounding the acute cerebral of ischemia area following vascular endothelial growth factor (VEGF) treatment using H-1-MRS imaging, and to evaluate the neuroprotective effects of VEGF.

DESIGN, TIME AND SETTING: Randomly controlled animal study. according to one-factor analysis of variance, was performed at the Shenzhen Hospital of Peking University and State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences from August 2003 to December 2005.

MATERIALS: Twelve healthy, adult, Sprague Dawley rats were used to establish an ischemia/reperfusion model through the use of middle cerebral artery Occlusion. The 4.7T superconducting nuclear magnetic resonance meter was provided by Brucker Company. VEGF(164) was purchased from Shenzhen Jingmei Bioengineering Co., Ltd. Titus anesthesia machine was purchased from Draeger Medical AG & Co. KG.

METHODS: The rats were randomly divided into model control (n = 6) and VEGF-injected (it = 6) groups. All animals received 60-minute middle cerebral artery Occlusion and 24-hour reperfusion. Lateral cerebral ventricle injection was performed by stercotaxic technique at respective time points. The VEGF group received 0.1 mu g/mu L VEGF (5 mu L), and the model group received the same amount of normal saline, once daily for 3 days.

MAIN OUTCOME MEASURES: Metabolic changes of N-acetyl-L-aspartic acid and lactic acid following cerebral ischemia and reperfusion were detected using H-1-MRS. and the ischemic volume was measured.

RESULTS: Twelve rats Were included in the final analysis. H-1-MRS results revealed that the ischemic volume increased in the control group compared with prior to injection (P < 0.01). In the control group. the level of N-acetyl-L-aspartic acid decreased significantly at 7 days after injection (P < 0.01), while lactic acid concentrations increased (P < 0.05). In the VEGF group. the level of N-acetyl-L-aspartic acid decreased slightly. as well as lactic acid concentrations (P < 0.05).

CONCLUSION: By means of H-1-MRS, results demonstrated that lactic acid and N-acetyl-L-aspartic acid concentrations decreased following treatment with VEGF. These results suggest that VEGF can provide prompt and Safe neuroprotection following ischemic stroke.

语种: 英语
所属项目编号: 20004086 ; 200404092 ; T152607
项目资助者: Scientific and Technological Research Projects of Shenzhen ; National Key Laboratory of the Chinese Academy of Sciences
WOS记录号: WOS:000261991400016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59871
Appears in Collections:北京大学深圳医院_神经内科_期刊论文

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作者单位: 1.Peking Univ, Dept Neurol, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Phys & Math, Wuhan 430074, Hubei Province, Peoples R China

Recommended Citation:
Yi, Li,Zhang, Haiou,Lei, Hao,et al. H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion[J]. NEURAL REGENERATION RESEARCH,2008,3(10):1116-1120.
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