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学科主题临床医学
H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion
Yi, Li1; Zhang, Haiou1; Lei, Hao2; Wei, Li2
关键词cerebral ischemia H-1-MRS neuroprotection vascular endothelial growth factor
刊名NEURAL REGENERATION RESEARCH
2008-10-01
3期:10页:1116-1120
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Neurosciences
研究领域[WOS]Cell Biology ; Neurosciences & Neurology
关键词[WOS]REDUCE INFARCTION VOLUME ; BRAIN-BARRIER DISRUPTION ; ARTERY OCCLUSION ; VEGF ; RAT ; DAMAGE ; MICE ; ISCHEMIA/REPERFUSION ; ANGIOGENESIS ; PERMEABILITY
英文摘要

BACKGROUND: It has become generally accepted that measuring N-acetyl-L-aspartic acid through the use of H-1-magnetic resonance spectroscopy (H-1-MRS) could be used to evaluate neuronal injury.

OBJECTIVE: To study metabolic changes of N-acetyl-L-aspartic acid surrounding the acute cerebral of ischemia area following vascular endothelial growth factor (VEGF) treatment using H-1-MRS imaging, and to evaluate the neuroprotective effects of VEGF.

DESIGN, TIME AND SETTING: Randomly controlled animal study. according to one-factor analysis of variance, was performed at the Shenzhen Hospital of Peking University and State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences from August 2003 to December 2005.

MATERIALS: Twelve healthy, adult, Sprague Dawley rats were used to establish an ischemia/reperfusion model through the use of middle cerebral artery Occlusion. The 4.7T superconducting nuclear magnetic resonance meter was provided by Brucker Company. VEGF(164) was purchased from Shenzhen Jingmei Bioengineering Co., Ltd. Titus anesthesia machine was purchased from Draeger Medical AG & Co. KG.

METHODS: The rats were randomly divided into model control (n = 6) and VEGF-injected (it = 6) groups. All animals received 60-minute middle cerebral artery Occlusion and 24-hour reperfusion. Lateral cerebral ventricle injection was performed by stercotaxic technique at respective time points. The VEGF group received 0.1 mu g/mu L VEGF (5 mu L), and the model group received the same amount of normal saline, once daily for 3 days.

MAIN OUTCOME MEASURES: Metabolic changes of N-acetyl-L-aspartic acid and lactic acid following cerebral ischemia and reperfusion were detected using H-1-MRS. and the ischemic volume was measured.

RESULTS: Twelve rats Were included in the final analysis. H-1-MRS results revealed that the ischemic volume increased in the control group compared with prior to injection (P < 0.01). In the control group. the level of N-acetyl-L-aspartic acid decreased significantly at 7 days after injection (P < 0.01), while lactic acid concentrations increased (P < 0.05). In the VEGF group. the level of N-acetyl-L-aspartic acid decreased slightly. as well as lactic acid concentrations (P < 0.05).

CONCLUSION: By means of H-1-MRS, results demonstrated that lactic acid and N-acetyl-L-aspartic acid concentrations decreased following treatment with VEGF. These results suggest that VEGF can provide prompt and Safe neuroprotection following ischemic stroke.

语种英语
WOS记录号WOS:000261991400016
项目编号20004086 ; 200404092 ; T152607
资助机构Scientific and Technological Research Projects of Shenzhen ; National Key Laboratory of the Chinese Academy of Sciences
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59871
专题北京大学深圳医院_神经内科
作者单位1.Peking Univ, Dept Neurol, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Phys & Math, Wuhan 430074, Hubei Province, Peoples R China
推荐引用方式
GB/T 7714
Yi, Li,Zhang, Haiou,Lei, Hao,et al. H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion[J]. NEURAL REGENERATION RESEARCH,2008,3(10):1116-1120.
APA Yi, Li,Zhang, Haiou,Lei, Hao,&Wei, Li.(2008).H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion.NEURAL REGENERATION RESEARCH,3(10),1116-1120.
MLA Yi, Li,et al."H-1-magnetic resonance spectroscopy of vascular endothelial growth factor-induced neuroprotection following acute cerebral ischemia and reperfusion".NEURAL REGENERATION RESEARCH 3.10(2008):1116-1120.
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