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学科主题: 临床医学
题名:
Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis
作者: Xu, Peng-Cheng1,2; Chen, Min1,2; Cui, Zhao1,2; Zhao, Ming-Hui1,2
关键词: Anti-neutrophil cytoplasmic antibodies ; Myeloperoxidase ; Microscopic polyangiitis ; Oxidation activity
刊名: RHEUMATOLOGY
发表日期: 2010-11-01
DOI: 10.1093/rheumatology/keq203
卷: 49, 期:11, 页:2068-2075
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: IGG SUBCLASS DISTRIBUTION ; WEGENERS-GRANULOMATOSIS ; SYSTEMIC VASCULITIS ; AUTOANTIBODIES ANCA ; HUMAN-NEUTROPHILS ; CHINESE PATIENTS ; FOLLOW-UP ; MPO-ANCA ; INHIBITION ; PROTEINASE-3
英文摘要:

Methods. MPO-ANCA was purified with MPO-affinity chromatography from plasma of 13 consecutive patients with MPO-ANCA-positive MPA. The titre, avidity, immunoglobulin G (IgG) subclasses and the ability to reverse the binding between ceruloplasmin and MPO of MPO-ANCA were tested using ELISA. The oxidation activity of MPO in the presence of MPO-ANCA was measured. The associations between the oxidation activity of MPO after binding MPO-ANCA and the clinical and immunological characteristics of MPO-ANCA were analysed.

Results. The MPO activity without binding to its autoantibodies was 1.521 (0.12) (expressed by A-values at 10 min, using o-phenylenediamine as substrate). After binding affinity-purified MPO-ANCA, the MPO activity ranged from 1.083 to 1.642. Correlation analysis showed that the oxidation activity of MPO after binding MPO-ANCA positively correlated with the level of ceruloplasmin binding on MPO in presence of MPO-ANCA (r = 0.617, P = 0.025) and negatively correlated with initial sera creatinine levels (r = -0.564, P = 0.045), BVAS (r = -0.735, P = 0.004), avidity of MPO-ANCA (r = -0.575, P = 0.040), levels of IgG2 (r = -0.610, P = 0.027) and IgG3 subclasses (r = -0.695, P = 0.008) of MPO-ANCA.

Conclusions. MPO-ANCA from different patients can influence MPO activity with different levels. The low oxidation activity of MPO after binding MPO-ANCA might be associated with more severe disease.

语种: 英语
所属项目编号: 30725034 ; 30972733
项目资助者: National Science Fund for Distinguished Young Scholars ; National Natural Science Fund
WOS记录号: WOS:000283120300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59886
Appears in Collections:北京大学第一临床医学院_大内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Med, Renal Div,Inst Nephrol, Beijing 100034, Peoples R China
2.Minist Hlth China, Key Lab Renal Dis, Beijing, Peoples R China

Recommended Citation:
Xu, Peng-Cheng,Chen, Min,Cui, Zhao,et al. Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis[J]. RHEUMATOLOGY,2010,49(11):2068-2075.
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