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学科主题: 基础医学
题名:
Apoptosis and activation of Erk1/2 and Akt in astrocytes postischemia
作者: Jiang, ZJ; Zhang, Y; Chen, XQ; Lam, PY; Yang, H; Xu, QY; Yu, ACH
关键词: astrocyte ; apoptosis ; Erk ; Akt ; ischemia
刊名: NEUROCHEMICAL RESEARCH
发表日期: 2003-06-01
卷: 28, 期:6, 页:831-837
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: CEREBRAL-ARTERY OCCLUSION ; NECROSIS-FACTOR-ALPHA ; REPERFUSION INJURY ; ISCHEMIC-INJURY ; CELL-DEATH ; BRAIN ; EXPRESSION ; RAT ; PHOSPHORYLATION ; INTERLEUKIN-6
英文摘要:

We have shown previously that in vitro ischemia could induce apoptosis in primary culture of astrocytes. In this paper we demonstrate that astrocytes in culture could undergo apoptosis during in vitro incubation postischemia. We also measured the changes of phosphorylated Erk1/2 (p-Erk1/2) and phosphorylated Akt (p-Akt) in order to determine whether these two pathways play a role in apoptosis. After 4 h in vitro ischemic incubation of cultured astrocytes, a limited amount of nuclear condensation was demonstrated by Hoechst 33342 staining. When ischemic incubation was halted and the cultures transferred to standard normoxic incubation (postischemia) conditions, DNA fragmentation and apoptosis were demonstrated by TUNEL and DNA laddering analysis. TUNEL-positive astrocytes began to appear at 6 h postischemia and increased in number from 12 h postischemia. Western blot analysis showed that both p-Erk1/2 and p-Akt were elevated in astrocytes subjected to 4 h of ischemia. Elevated p-Erk1/2 levels were sustained during the postischemia incubation for 12 h and decreased significantly afterward, but did not return to the levels in the control cultures that did not experience ischemic insult. In contrast, the p-Akt level continued to increase at 6 and 12 h postischemia before declining significantly. The changes in p-Erk1/2 and p-Akt correlated well with the appearance of apoptotic astrocytes in the culture.

语种: 英语
WOS记录号: WOS:000182099900006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59891
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Capital Univ Med Sci, Beijing Inst Neurosci, Beijing, Peoples R China
4.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Jiang, ZJ,Zhang, Y,Chen, XQ,et al. Apoptosis and activation of Erk1/2 and Akt in astrocytes postischemia[J]. NEUROCHEMICAL RESEARCH,2003,28(6):831-837.
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