IR@PKUHSC  > 北京大学基础医学院  > 北京大学衰老研究中心
学科主题基础医学
Transcriptional activation of the senescence regulator Lsh by E2F1
Niu, Jing; Chen, Tianda; Han, Limin; Wang, Pan; Li, Na; Tong, Tanjun
关键词Lsh E2F1 Transcriptional regulation Cellular senescence
刊名MECHANISMS OF AGEING AND DEVELOPMENT
2011-04-01
DOI10.1016/j.mad.2011.03.004
132期:4页:180-186
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Geriatrics & Gerontology
资助者National Basic Research Programs of China ; National Natural Science Foundation of China ; National Basic Research Programs of China ; National Natural Science Foundation of China
研究领域[WOS]Cell Biology ; Geriatrics & Gerontology
关键词[WOS]HUMAN-DIPLOID FIBROBLASTS ; CELL-PROLIFERATION ; TUMOR-SUPPRESSOR ; GENE-EXPRESSION ; FAMILY-MEMBER ; DNA-SYNTHESIS ; BINDING-SITE ; SNF2 FAMILY ; IN-VIVO ; APOPTOSIS
英文摘要

Lsh, a protein related to the SNF2 family of chromatin-remodeling ATPases, is a major epigenetic regulator that is essential for DNA methylation and histone acetylation at repetitive elements. Lsh represses endogenous p16(INK4a) expression by recruiting HDAC to the p(16IN4Ka) promoter, which in turn delays cell senescence. However, the molecular mechanisms that govern loss of Lsh expression during cellular senescence have yet to be elucidated. Here we investigate the transcriptional regulation of the human Lsh promoter. We find that the minimal Lsh promoter is located between positions -216 and -119 relative to the transcription start site, and contains two putative E2F binding sites. Ectopic E2F1 increases expression of Lsh at both transcriptional and translational levels. E2F1 physically interacts with the Lsh promoter by binding to each of the two putative binding sites and transactivates the Lsh promoter. E2F1 also induces Lsh protein expression and transactivates the Lsh promoter in 2BS cells. At the same time, E2F1-induced Lsh promoter activity is reduced in senescent cells compared to young cells. These results indicate that E2F1 plays a crucial role in transcriptional control of the human Lsh gene and the decrease of Lsh expression in senescent cells is related to the repression of E2F1. Published by Elsevier Ireland Ltd.

语种英语
所属项目编号2007CB507400 ; 30973146
资助者National Basic Research Programs of China ; National Natural Science Foundation of China ; National Basic Research Programs of China ; National Natural Science Foundation of China
WOS记录号WOS:000291628000006
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59894
Collection北京大学基础医学院_北京大学衰老研究中心
作者单位Peking Univ, Hlth Sci Ctr, Res Ctr Aging, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Niu, Jing,Chen, Tianda,Han, Limin,et al. Transcriptional activation of the senescence regulator Lsh by E2F1[J]. MECHANISMS OF AGEING AND DEVELOPMENT,2011,132(4):180-186.
APA Niu, Jing,Chen, Tianda,Han, Limin,Wang, Pan,Li, Na,&Tong, Tanjun.(2011).Transcriptional activation of the senescence regulator Lsh by E2F1.MECHANISMS OF AGEING AND DEVELOPMENT,132(4),180-186.
MLA Niu, Jing,et al."Transcriptional activation of the senescence regulator Lsh by E2F1".MECHANISMS OF AGEING AND DEVELOPMENT 132.4(2011):180-186.
Files in This Item: Download All
File Name/Size DocType Version Access License
1-s2.0-S004763741100(543KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Niu, Jing]'s Articles
[Chen, Tianda]'s Articles
[Han, Limin]'s Articles
百度学术
百度学术Similar articles in
[Niu, Jing]'s Articles
[Chen, Tianda]'s Articles
[Han, Limin]'s Articles
必应学术
必应学术Similar articles in
[Niu, Jing]'s Articles
[Chen, Tianda]'s Articles
[Han, Limin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0047637411000431-main.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.