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RNAi-mediated knockdown of pituitary tumor-transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
Huang, S. Q.1,2,3,4; Liao, Q. J.1,2; Wang, X. W.1,2; Xin, D. Q.3,4; Chen, S. X.1,2; Wu, Q. J.1,2; Ye, G.1,2
关键词Pttg1 Prostate Cancer Cell Proliferation Transwell Invasion Assay Anticancer Drug Resistance
刊名BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
2012-11-01
DOI10.1590/S0100-879X2012007500126
45期:11页:995-1001
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology ; Medicine, Research & Experimental
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Life Sciences & Biomedicine - Other Topics ; Research & Experimental Medicine
关键词[WOS]INCREASED EXPRESSION ; POOR-PROGNOSIS ; ACTIVATION ; IDENTIFICATION ; ANGIOGENESIS ; CARCINOMA ; TARGET ; DOMAIN
英文摘要

Pituitary tumor-transforming gene-1 (PTTG1) is a proto-oncogene that promotes tumorigenesis and metastasis in numerous cell types and is overexpressed in a variety of human tumors. We have demonstrated that PTTG1 expression was up-regulated in both human prostate cancer specimens and prostate cancer cell lines. For a more direct assessment of the function of PTTG1 in prostate tumorigenesis, RNAi-mediated knockdown was used to selectively decrease PTTG1 expression in PC3 human prostate tumor cells. After three weeks of selection, colonies stably transfected with PTTG1-targeted RNAi (the knockdown PC3 cell line) or empty vector (the control PC3 cell line) were selected and expanded to investigate the role of PTTG1 expression in PC3 cell growth and invasion. Cell proliferation rate was significantly slower (28%) in the PTTG1 knockdown line after 6 days of growth as indicated by an MTT cell viability assay (P < 0.05). Similarly, a soft agar colony formation assay revealed significantly fewer (66.7%) PTTG1 knockdown PC3 cell colonies than control colonies after three weeks of growth. In addition, PTTG1 knockdown resulted in cell cycle arrest at G1 as indicated by fluorescence-activated cell sorting. The PTTG1 knockdown PC3 cell line also exhibited significantly reduced migration through Matrigel in a transwell assay of invasive potential, and down-regulation of PTTG1 could lead to increased sensitivity of these prostate cancer cells to a commonly used anticancer drug, taxol. Thus, PTTG1 expression is crucial for PC3 cell proliferation and invasion, and could be a promising new target for prostate cancer therapy.

语种英语
所属项目编号81172443
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000309470400001
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59896
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
3.Third Mil Med Univ, Xinqiao Hosp, Dept Urol, Chongqing 400037, Peoples R China
4.Third Mil Med Univ, Xinqiao Hosp, Ctr Nephrol, Chongqing 400037, Peoples R China
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Huang, S. Q.,Liao, Q. J.,Wang, X. W.,et al. RNAi-mediated knockdown of pituitary tumor-transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells[J]. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH,2012,45(11):995-1001.
APA Huang, S. Q..,Liao, Q. J..,Wang, X. W..,Xin, D. Q..,Chen, S. X..,...&Ye, G..(2012).RNAi-mediated knockdown of pituitary tumor-transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells.BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH,45(11),995-1001.
MLA Huang, S. Q.,et al."RNAi-mediated knockdown of pituitary tumor-transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells".BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH 45.11(2012):995-1001.
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