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学科主题: 临床医学
题名:
Analysis of phospholipids by NACE with on-line ESI-MS
作者: Gao, Fei; Zhang, Zhengxiang; Fu, Xiaofang; Li, Wei; Wang, Tao; Liu, Huwei
关键词: MS ; NACE ; phospholipids
刊名: ELECTROPHORESIS
发表日期: 2007-05-01
DOI: 10.1002/elps.200600533
卷: 28, 期:9, 页:1418-1425
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: NONAQUEOUS CAPILLARY-ELECTROPHORESIS ; IONIZATION MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; INDIRECT ULTRAVIOLET DETECTION ; LASER-INDUCED FLUORESCENCE ; ELECTROSPRAY-IONIZATION ; QUANTITATIVE-ANALYSIS ; SIGNAL-TRANSDUCTION ; SEPARATION ; SURFACTANT
英文摘要:

A hyphenated method of nonaqueous capillary electrophoresis coupled to electrospray ionization mass spectrometry (NACE-ESI-MS) is described for the simultaneous analysis of phospholipids. The best results were obtained with a mixed solution of methanol/ACN (40:60 v/v) containing 20 mM ammonium acetate and 0.5% acetic acid, under the applied voltage of 30 kV and capillary temperature of 25 degrees C. ESI-MS measurements were performed in the negative mode with methanol/ACN (40:60 v/v) containing 50 mM ammonium acetate as sheath liquid at a flow rate of 2 mu L/min. Different phospholipid classes have been successfully separated within 16 min, and the molecular species of every single class have been identified by using MS2 or MS3, which generates characteristic fragments through CID. The developed method has been applied to analyze the phospholipids extracted from rat peritoneal surface and the molecular species of phospholipid classes are presented.

语种: 英语
WOS记录号: WOS:000246557400016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59900
Appears in Collections:北京大学第三临床医学院_肾内科_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 3, Div Nephrol, Beijing 100871, Peoples R China

Recommended Citation:
Gao, Fei,Zhang, Zhengxiang,Fu, Xiaofang,et al. Analysis of phospholipids by NACE with on-line ESI-MS[J]. ELECTROPHORESIS,2007,28(9):1418-1425.
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