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学科主题临床医学
Anti-inflammatory effect of PPAR gamma in cultured human mesangial cells
Xiong, ZY; Huang, HC; Li, JZ; Guan, YF; Wang, HY
关键词Peroxisome Proliferator-activator Receptor-gamma Mesangial Cell Inflammation Agonist Tumor Necrosis Factor-alpha
刊名RENAL FAILURE
2004
DOI10.1081/JDI-200031747
26期:5页:497-505
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]ACTIVATED RECEPTOR-GAMMA ; EXPRESSION ; AGONISTS ; TROGLITAZONE ; INHIBIT ; GLOMERULOSCLEROSIS ; DIFFERENTIATION ; NEPHROPATHY ; PROTECTS ; LIGANDS
英文摘要

Our aim is to investigate whether peroxisome proliferator-activator receptor-gamma (PPARgamma) expression was altered in human mesangial cells under inflammatory stress and whether PPARgamma could retard the inflammatory responses. Based on cultured human mesangial cell lines (HMCLs), PPARgamma expressions at protein and mRNA levels were observed by Western blot analysis and reverse transcriptase polymerase chain reaction. Informatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay. Our results demonstrated that PPARgamma protein expression was dramatically increased in HMCLs stimulated by IL-1beta (10 ng/mL). The levels of IL-6 and TNF-alpha in HMCL supernatants, protein, and mRNA expressions of PPARgamma in IL-1beta challenge cells were significantly increased more than those in untreated cells. Importantly, PPARgamma agonists troglitazone, rosiglitazone, and 15-deoxy-Delta(12,14)-prosglandin J(2) significantly decreased the up expression of TNF-a and IL-6 in HMCL supernatants stimulated by IL-1beta. Furthermore, troglitazone downregulated TNF-alpha and IL-6 mRNA expression from IL-1beta challenge HMCLs. Our data suggest that PPARgamma plays an important role in mesangial cells responding to inflammatory stress. PPARgamma may prove to be a pharmacological target in glomerulonephritis.

语种英语
WOS记录号WOS:000224501400004
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59950
专题北京大学第一临床医学院_肾脏内科
作者单位1.Peking Univ, Div Renal, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Inst Nephrol, Beijing 100034, Peoples R China
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Xiong, ZY,Huang, HC,Li, JZ,et al. Anti-inflammatory effect of PPAR gamma in cultured human mesangial cells[J]. RENAL FAILURE,2004,26(5):497-505.
APA Xiong, ZY,Huang, HC,Li, JZ,Guan, YF,&Wang, HY.(2004).Anti-inflammatory effect of PPAR gamma in cultured human mesangial cells.RENAL FAILURE,26(5),497-505.
MLA Xiong, ZY,et al."Anti-inflammatory effect of PPAR gamma in cultured human mesangial cells".RENAL FAILURE 26.5(2004):497-505.
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