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学科主题基础医学
Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin
Jiang, Changtao1; Zhang, Heng1; Zhang, Weizhen1; Kong, Wei1; Zhu, Yi1; Zhang, Hongquan2; Xu, Qingbo5; Li, Yin1; Wang, Xian1,3,4
关键词adipose-vascular interaction insulin resistance cytoskeleton
刊名AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
2009-12-01
DOI10.1152/ajpcell.00304.2009
297期:6页:C1466-C1476
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]ADIPOSE-TISSUE ; INSULIN-RESISTANCE ; DIABETES-MELLITUS ; BETA-3 INTEGRINS ; RHO-GTPASES ; IN-VITRO ; ATHEROSCLEROSIS ; ACTIVATION ; EXPRESSION ; ADHESION
英文摘要

Jiang C, Zhang H, Zhang W, Kong W, Zhu Y, Zhang H, Xu Q, Li Y, Wang X. Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin. Am J Physiol Cell Physiol 297: C1466-C1476, 2009. First published October 14, 2009; doi:10.1152/ajpcell.00304.2009.-Adipokines may represent a mechanism linking insulin resistance to cardiovascular disease. We showed previously that homocysteine (Hcy), an independent risk factor for cardiovascular disease, can induce the expression and secretion of resistin, a novel adipokine, in vivo and in vitro. Since vascular smooth muscle cell (VSMC) migration is a key event in vascular disease, we hypothesized that adipocyte-derived resistin is involved in Hcy-induced VSMC migration. To confirm our hypothesis, Sprague-Dawley rat aortic SMCs were cocultured with Hcy-stimulated primary rat epididymal adipocytes or treated directly with increasing concentrations of resistin for up to 24 h. Migration of VSMCs was investigated. Cytoskeletal structure and cytoskeleton-related proteins were also detected. The results showed that Hcy (300-500 mu M) increased migration significantly in VSMCs cocultured with adipocytes but not in VSMC cultured alone. Resistin alone also significantly increased VSMC migration in a time- and concentration-dependent manner. Resistin small interfering RNA (siRNA) significantly attenuated VSMC migration in the coculture system, which indicated that adipocyte-derived resistin mediates Hcy-induced VSMC migration. On cell spreading assay, resistin induced the formation of focal adhesions near the plasma membrane, which suggests cytoskeletal rearrangement via an alpha(5)beta(1)-integrin-focal adhesion kinase/paxillin-Ras-related C3 botulinum toxin substrate 1 (Rac1) pathway. Our data demonstrate that Hcy promotes VSMC migration through a paracrine or endocrine effect of adipocyte-derived resistin, which provides further evidence of the adipose-vascular interaction in metabolic disorders. The migratory action exerted by resistin on VSMCs may account in part for the increased incidence of restenosis in diabetic patients.

语种英语
WOS记录号WOS:000272156100016
项目编号2006CB503802 ; 30730042 ; 30821001 ; 30700376 ; 30971085 ; 307001 ; 7083110
资助机构Major National Basic Research Program of the People&prime ; s Republic of China ; National Natural Science Foundation of China ; Key Grant Project of Chinese Ministry of Education ; Beijing Natural Science Foundation ; Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry ; Chang Jiang Scholars Program
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/59951
专题北京大学基础医学院
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Dept Anat & Histol Embryol, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
5.Kings Coll London, British Heart Fdn Ctr, Div Cardiovasc, London WC2R 2LS, England
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GB/T 7714
Jiang, Changtao,Zhang, Heng,Zhang, Weizhen,et al. Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2009,297(6):C1466-C1476.
APA Jiang, Changtao.,Zhang, Heng.,Zhang, Weizhen.,Kong, Wei.,Zhu, Yi.,...&Wang, Xian.(2009).Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,297(6),C1466-C1476.
MLA Jiang, Changtao,et al."Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 297.6(2009):C1466-C1476.
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