北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin
作者: Jiang, Changtao1; Zhang, Heng1; Zhang, Weizhen1; Kong, Wei1; Zhu, Yi1; Zhang, Hongquan2; Xu, Qingbo5; Li, Yin1; Wang, Xian1,3,4
关键词: adipose-vascular interaction ; insulin resistance ; cytoskeleton
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
发表日期: 2009-12-01
DOI: 10.1152/ajpcell.00304.2009
卷: 297, 期:6, 页:C1466-C1476
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Physiology
研究领域[WOS]: Cell Biology ; Physiology
关键词[WOS]: ADIPOSE-TISSUE ; INSULIN-RESISTANCE ; DIABETES-MELLITUS ; BETA-3 INTEGRINS ; RHO-GTPASES ; IN-VITRO ; ATHEROSCLEROSIS ; ACTIVATION ; EXPRESSION ; ADHESION
英文摘要:

Jiang C, Zhang H, Zhang W, Kong W, Zhu Y, Zhang H, Xu Q, Li Y, Wang X. Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin. Am J Physiol Cell Physiol 297: C1466-C1476, 2009. First published October 14, 2009; doi:10.1152/ajpcell.00304.2009.-Adipokines may represent a mechanism linking insulin resistance to cardiovascular disease. We showed previously that homocysteine (Hcy), an independent risk factor for cardiovascular disease, can induce the expression and secretion of resistin, a novel adipokine, in vivo and in vitro. Since vascular smooth muscle cell (VSMC) migration is a key event in vascular disease, we hypothesized that adipocyte-derived resistin is involved in Hcy-induced VSMC migration. To confirm our hypothesis, Sprague-Dawley rat aortic SMCs were cocultured with Hcy-stimulated primary rat epididymal adipocytes or treated directly with increasing concentrations of resistin for up to 24 h. Migration of VSMCs was investigated. Cytoskeletal structure and cytoskeleton-related proteins were also detected. The results showed that Hcy (300-500 mu M) increased migration significantly in VSMCs cocultured with adipocytes but not in VSMC cultured alone. Resistin alone also significantly increased VSMC migration in a time- and concentration-dependent manner. Resistin small interfering RNA (siRNA) significantly attenuated VSMC migration in the coculture system, which indicated that adipocyte-derived resistin mediates Hcy-induced VSMC migration. On cell spreading assay, resistin induced the formation of focal adhesions near the plasma membrane, which suggests cytoskeletal rearrangement via an alpha(5)beta(1)-integrin-focal adhesion kinase/paxillin-Ras-related C3 botulinum toxin substrate 1 (Rac1) pathway. Our data demonstrate that Hcy promotes VSMC migration through a paracrine or endocrine effect of adipocyte-derived resistin, which provides further evidence of the adipose-vascular interaction in metabolic disorders. The migratory action exerted by resistin on VSMCs may account in part for the increased incidence of restenosis in diabetic patients.

语种: 英语
所属项目编号: 2006CB503802 ; 30730042 ; 30821001 ; 30700376 ; 30971085 ; 307001 ; 7083110
项目资助者: Major National Basic Research Program of the People&prime ; s Republic of China ; National Natural Science Foundation of China ; Key Grant Project of Chinese Ministry of Education ; Beijing Natural Science Foundation ; Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry ; Chang Jiang Scholars Program
WOS记录号: WOS:000272156100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/59951
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Dept Anat & Histol Embryol, Sch Basic Med Sci, Beijing 100191, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
5.Kings Coll London, British Heart Fdn Ctr, Div Cardiovasc, London WC2R 2LS, England

Recommended Citation:
Jiang, Changtao,Zhang, Heng,Zhang, Weizhen,et al. Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2009,297(6):C1466-C1476.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jiang, Changtao]'s Articles
[Zhang, Heng]'s Articles
[Zhang, Weizhen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jiang, Changtao]‘s Articles
[Zhang, Heng]‘s Articles
[Zhang, Weizhen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace